|
|
|
|
||
Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot2936
Abstract
Full Text
Ecdysone as Regulator of Inducible Gene Expression in Mammalian Cells
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3186
Abstract
Full Text
Recovery of DNA from Agarose Gels: Electrophoresis onto DEAE-cellulose Membranes
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3214
Abstract
Full Text
Markers for Pulsed-field Gel Electrophoresis
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3222
Abstract
Full Text
Isolation of High-molecular-weight DNA from Mammalian Cells Using Formamide
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3225
Abstract
Full Text
Preparation of DNA for Pulsed-field Gel Electrophoresis: Isolation of Intact DNA from Yeast
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3235
Abstract
Full Text
Preparation of Double-stranded (Replicative Form) Bacteriophage M13 DNA
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3281
Abstract
Full Text
Large-scale Preparation of Single-stranded and Double-stranded Bacteriophage M13 DNA
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3284
Abstract
Full Text
Screening an Unamplified Cosmid Library by Hybridization: Plating the Library onto Filters
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3289
Abstract
Full Text
Working with Yeast Artificial Chromosomes
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3297
Abstract
Full Text
Growth of S. cerevisiae and Preparation of DNA
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3298
Abstract
Full Text
Small-scale Preparations of Yeast DNA
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3299
Abstract
Full Text
Construction of cDNA Libraries • Stage 4: Attachment of Linkers or Adaptors
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3310
Abstract
Full Text
Removal of Cross-reactive Antibodies from Antiserum: Affinity Chromatography
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3340
Abstract
Full Text
Site-specific Mutagenesis by Overlap Extension
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3468
Abstract
Full Text
Mixed Oligonucleotide-primed Amplification of cDNA (MOPAC)
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3484
Abstract
Full Text
Inverse PCR
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3487
Abstract
Full Text
Purification of Radiolabeled Oligonucleotides by Precipitation with Ethanol
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3490
Abstract
Full Text
Rapid Isolation of Mammalian DNA
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3514
Abstract
Full Text
Random Priming: Radiolabeling of Purified DNA Fragments by Extension of Random Oligonucleotides
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3542
Abstract
Full Text
Random Priming: Radiolabeling of DNA by Extension of Random Oligonucleotides in the Presence of Melted Agarose
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3543
Abstract
Full Text
Synthesis of Single-stranded DNA Probes of Heterogeneous Length from Bacteriophage M13 Templates
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3546
Abstract
Full Text
Labeling 3' Termini of Double-stranded DNA Using the Klenow Fragment of E. coli DNA Polymerase I
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3551
Abstract
Full Text
Construction of cDNA Libraries • Stage 2: Second-strand Synthesis
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3560
Abstract
Full Text
Labeling of Synthetic Oligonucleotides Using the Klenow Fragment of E. coli DNA Polymerase I
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3577
Abstract
Full Text
Autoradiography and Reading of Sequencing Gels
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3588
Abstract
Full Text
Generation of Bidirectional Sets of Deletion Mutants by Digestion with BAL 31 Nuclease
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3589
Abstract
Full Text
Expression of Cloned Genes in E. coli Using the Bacteriophage T7 Promoter
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3597
Abstract
Full Text
Assay for Luciferase in Extracts of Mammalian Cells
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3642
Abstract
Full Text
Exon Trapping and Amplification • Stage 5: Analysis of Clones
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3651
Abstract
Full Text
Expression of Secreted Foreign Proteins Using the Alkaline Phosphatase Promoter (phoA) and Signal Sequence
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3654
Abstract
Full Text
Purification of Fusion Proteins by Affinity Chromatography on Glutathione Agarose
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3655
Abstract
Full Text
Preparation of Single-stranded Bacteriophage M13 DNA
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3686
Abstract
Full Text
Isolation of DNA from Mammalian Cells Grown in 96-well Microtiter Plates
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3695
Abstract
Full Text
Detection of DNA in Polyacrylamide Gels by Staining
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3718
Abstract
Full Text
Northern Hybridization
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3723
Abstract
Full Text
Purification of Radiolabeled Oligonucleotides by Size-exclusion Chromatography
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3727
Abstract
Full Text
Construction of cDNA Libraries • Stage 5: Fractionation of cDNA by Gel Filtration through Sepharose CL-4B
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3730
Abstract
Full Text
Exon Trapping and Amplification • Stage 2: Electroporation of the Library into COS-7 Cells
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3733
Abstract
Full Text
Exon Trapping and Amplification • Stage 3: Harvesting the mRNA
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3734
Abstract
Full Text
Oligonucleotide-directed Mutagenesis by Elimination of a Unique Restriction Site (USE Mutagenesis)
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3742
Abstract
Full Text
DNA Transfection by Electroporation
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3751
Abstract
Full Text
Detection of Protein-Protein Interactions Using the GST Fusion Protein Pulldown Technique
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3757
Abstract
Full Text
Screening Expression Libraries Constructed in Bacteriophage 
Vectors
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3765
Abstract
Full Text
Removal of Cross-reactive Antibodies from Antiserum: Incubation with E. coli Lysate
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3767
Abstract
Full Text
Preparation of Lysates Containing Fusion Proteins Encoded by Bacteriophage Lysogens: Lysis of Bacterial Colonies
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3769
Abstract
Full Text
Preparation of Lysates Containing Fusion Proteins Encoded by Bacteriophage : Lytic Infections on Agar Plates
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3770
Abstract
Full Text
Preparation of Lysates Containing Fusion Proteins Encoded by Bacteriophage : Lytic Infections in Liquid Medium
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3771
Abstract
Full Text
Removal of Cross-reactive Antibodies from Antiserum: Pseudoscreening
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3772
Abstract
Full Text
Identifying DNA-binding Proteins in Bacteriophage Expression Libraries
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3785
Abstract
Full Text
Preparing Denatured Templates for Sequencing by Dideoxy-mediated Chain Termination
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3787
Abstract
Full Text
Cycle Sequencing: Dideoxy-mediated Sequencing Reactions Using PCR and End-labeled Primers
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3791
Abstract
Full Text
Preparation of Denaturing Polyacrylamide Gels
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3793
Abstract
Full Text
Loading and Running DNA Sequencing Gels
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3794
Abstract
Full Text
Preparation of Electrolyte Gradient Gels
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3795
Abstract
Full Text
Dideoxy-mediated Sequencing Reactions Using Bacteriophage T7 DNA Polymerase (Sequenase)
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3801
Abstract
Full Text
Dideoxy-mediated Sequencing Reactions Using the Klenow Fragment of E. coli DNA Polymerase I and Single-stranded DNA Templates
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3802
Abstract
Full Text
Dideoxy-mediated Sequencing of DNA Using Taq DNA Polymerase
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3803
Abstract
Full Text
Chemical Sequencing
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3804
Abstract
Full Text
Preparation of Denaturing Polyacrylamide Gels Containing Formamide
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3807
Abstract
Full Text
Preparation of Uracil-containing Single-stranded Bacteriophage M13 DNA
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3811
Abstract
Full Text
In Vitro Mutagenesis Using Double-stranded DNA Templates: Selection of Mutants with DpnI
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3813
Abstract
Full Text
Detection of Mutations by Single-strand Conformational Polymorphism
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3815
Abstract
Full Text
Oligonucleotide-directed Mutagenesis of Single-stranded DNA
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3817
Abstract
Full Text
Probing Protein Interactions Using GFP and FRET • Stage 3: FLIM-FRET Measurements
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3822
Abstract
Full Text
The Basic Polymerase Chain Reaction
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3824
Abstract
Full Text
Purification of PCR Products in Preparation for Cloning
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3825
Abstract
Full Text
Removal of Oligonucleotides and Excess dNTPs from Amplified DNA by Ultrafiltration
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3826
Abstract
Full Text
Blunt-end Cloning of PCR Products
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3830
Abstract
Full Text
Cloning PCR Products into T Vectors
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3834
Abstract
Full Text
Cloning PCR Products by Addition of Restriction Sites to the Termini of Amplified DNA
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3835
Abstract
Full Text
Genetic Engineering with PCR
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3836
Abstract
Full Text
Amplification of cDNA Generated by Reverse Transcription of mRNA
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3837
Abstract
Full Text
Rapid Characterization of DNAs Cloned in Prokaryotic Vectors
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3840
Abstract
Full Text
Long PCR
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3841
Abstract
Full Text
Quantitative PCR
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3842
Abstract
Full Text
Differential Display-PCR
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3844
Abstract
Full Text
Synthesis of Single-stranded RNA Probes by In Vitro Transcription
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3847
Abstract
Full Text
Radiolabeling of DNA Probes by the Polymerase Chain Reaction
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3848
Abstract
Full Text
Labeling 3' Termini of Double-stranded DNA with Bacteriophage T4 DNA Polymerase
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3852
Abstract
Full Text
End Labeling Protruding 3' Termini of Double-stranded DNA with [
-32P]Cordycepin 5'-Triphosphate or [-32P]dideoxyATP
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3853
Abstract
Full Text
Radiolabeling of Subtracted cDNA Probes by Random Oligonucleotide Extension
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3860
Abstract
Full Text
Dephosphorylation of DNA Fragments with Alkaline Phosphatase
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3863
Abstract
Full Text
Phosphorylation of DNA Molecules with Protruding 5'-Hydroxyl Termini
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3864
Abstract
Full Text
Rapid Amplification of 3' cDNA Ends (3'-RACE)
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3865
Abstract
Full Text
Synthesis of cDNA Probes from mRNA Using Random Oligonucleotide Primers
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3868
Abstract
Full Text
Synthesis of Radiolabeled, Subtracted cDNA Probes Using Oligo(dT) as a Primer
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3869
Abstract
Full Text
DNA Transfection Mediated by Lipofection
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3870
Abstract
Full Text
Calcium-phosphate-mediated Transfection of Eukaryotic Cells with Plasmid DNAs
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3871
Abstract
Full Text
Calcium-phosphate-mediated Transfection of Cells with High-molecular-weight Genomic DNA
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3872
Abstract
Full Text
DNA Transfection by Biolistics
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3874
Abstract
Full Text
DNA Transfection Using Polybrene
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3875
Abstract
Full Text
Transfection Mediated by DEAE-Dextran: High-efficiency Method
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3876
Abstract
Full Text
Phosphorylating the 5' Termini of Oligonucleotides
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3880
Abstract
Full Text
Purification of Radiolabeled Oligonucleotides by Precipitation with Cetylpyridinium Bromide
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3882
Abstract
Full Text
Hybridization of Oligonucleotide Probes in Aqueous Solutions: Washing in Buffers Containing Quaternary Ammonium Salts
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3883
Abstract
Full Text
Empirical Measurement of Melting Temperature
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3886
Abstract
Full Text
Two-hybrid Systems • Stage 2: Selecting an Interactor
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3888
Abstract
Full Text
Two-hybrid Systems
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3889
Abstract
Full Text
Probing Protein Interactions Using GFP and FRET • Stage 2: Cell Preparation for FLIM-FRET Analysis
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3893
Abstract
Full Text
     Copyright © 2010 by Cold Spring Harbor Laboratory Press. Online ISSN: 1559-6095 Terms of Service |