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Cover Illustration: DiI staining of mouse embryo. Photo courtesy of David A. Crotty and Scott E. Fraser.
Epitope Mapping by Competition Assay
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4277
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Epitope Mapping Using Synthetic Biotin-Labeled Peptides
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4278
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Storage of Sera
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4279
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Storage of Tissue Culture Supernatants
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4280
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Storage of Ascitic Fluid
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4281
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Labeling Antibodies with Fluorochromes
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4285
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Labeling Antibodies with Biotin
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4286
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Labeling Antibodies with Iodine
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4287
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Labeling Monoclonal Antibodies by Biosynthesis
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4288
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Visualizing Early Embryo Implantation Sites by Dye Injection
Sudhansu K. Dey
CSH Protocols; 2006; doi:10.1101/pdb.prot4361
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Vasectomy for Generation of Sterile Males: Access via Abdominal Wall
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4376
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Vasectomy for Generation of Sterile Males: Access via Scrotal Sac
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4377
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Oviduct Transfer
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4379
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Uterine Transfer
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4380
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Caesarean Section and Fostering
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4381
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Transplantation of Tissues Under the Kidney Capsule
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4382
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Subcutaneous Injection of Embryonic Stem (ES) Cells
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4383
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Partial Hepatectomy
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4384
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Splenectomy
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4385
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Nephrectomy
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4386
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Castration
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4387
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Blood Collection by Tail Bleeding
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4388
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Differentiating Embryonic Stem (ES) Cells into Embryoid Bodies
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4405
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Culturing Trophoblast Stem (TS) Cell Lines
Janet Rossant
CSH Protocols; 2006; doi:10.1101/pdb.prot4406
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Derivation of Trophoblast Stem (TS) Cell Lines from Blastocysts
Janet Rossant
CSH Protocols; 2006; doi:10.1101/pdb.prot4407
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Electroporating DNA into Embryonic Stem (ES) Cells and Selection Methods
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4409
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Isolating Individual Embryonic Stem (ES) Cell Colonies by Picking
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4410
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Freezing Embryonic Stem (ES) Cells in 96-Well Plates
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4411
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Rapid Preparation of DNA from Cells in 96-Well Tissue Culture Dishes
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4413
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Preparation of DNA from Cells in 24-Well Tissue Culture Plates
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4414
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Microscopic Analysis of Mitochondrial Transmembrane Potential (
m)
Shailaja Kasibhatla, Gustavo P. Amarante-Mendes, Deborah Finucane, Thomas Brunner, Ella Bossy-Wetzel, and Douglas R. Green
CSH Protocols; 2006; doi:10.1101/pdb.prot4462
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Analysis of TUNEL Staining by Flow Cytometry to Detect Apoptosis
Shailaja Kasibhatla, Gustavo P. Amarante-Mendes, Deborah Finucane, Thomas Brunner, Ella Bossy-Wetzel, and Douglas R. Green
CSH Protocols; 2006; doi:10.1101/pdb.prot4463
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Isolation of Fibroblasts from Mammalian Embryos
Anne Goldman
CSH Protocols; 2006; doi:10.1101/pdb.prot4474
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Isolation of Fibroblasts from Chicken Embryos
Anne Goldman
CSH Protocols; 2006; doi:10.1101/pdb.prot4475
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Lymphocyte Isolation and Culture
Gretchen J. Darlington
CSH Protocols; 2006; doi:10.1101/pdb.prot4480
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Epstein-Barr Virus Transformation of Lymphoblasts
Gretchen J. Darlington
CSH Protocols; 2006; doi:10.1101/pdb.prot4481
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Preparation of Mouse Embryonic Fibroblast (MEF) Feeder Plates
Linda C. Samuelson and Joseph M. Metzger
CSH Protocols; 2006; doi:10.1101/pdb.prot4483
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Propagation of Pluripotent Mouse Embryonic Stem (ES) Cells
Linda C. Samuelson and Joseph M. Metzger
CSH Protocols; 2006; doi:10.1101/pdb.prot4484
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Differentiation of Embryonic Stem (ES) Cells Using the Hanging Drop Method
Linda C. Samuelson and Joseph M. Metzger
CSH Protocols; 2006; doi:10.1101/pdb.prot4485
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G1/S Phase Synchronization using Double Thymidine Synchronization
Paul J. Galgano and Carl L. Schildkraut
CSH Protocols; 2006; doi:10.1101/pdb.prot4487
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Cell Synchronization Using Centrifugal Elutriation
Paul J. Galgano and Carl L. Schildkraut
CSH Protocols; 2006; doi:10.1101/pdb.prot4490
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Propidium Iodide (PI) Uptake Assay to Detect Apoptosis
Shailaja Kasibhatla, Gustavo P. Amarante-Mendes, Deborah Finucane, Thomas Brunner, Ella Bossy-Wetzel, and Douglas R. Green
CSH Protocols; 2006; doi:10.1101/pdb.prot4495
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TUNEL Staining of Tissue Sections to Detect Apoptosis
Shailaja Kasibhatla, Gustavo P. Amarante-Mendes, Deborah Finucane, Thomas Brunner, Ella Bossy-Wetzel, and Douglas R. Green
CSH Protocols; 2006; doi:10.1101/pdb.prot4496
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