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Cover Illustration: Injection of fluorescently labeled mRNA into a stage-4 syncytial blastoderm Drosophila embryo. Image from Fluorescent Reagents for Live Cell Imaging and Their Introduction into Cells (doi:10.1101/pdb.ip20).
Growing Adherent Cells on Coverslips or Multiwell Slides
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4289
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Growing Adherent Cells Directly on Tissue Dishes
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4290
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Attaching Suspension Cells to Slides Using a Cytocentrifuge
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4291
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Attaching Suspension Cells to Slides Using Chemical Linkers
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4292
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Fixing Attached Cells in Organic Solvents
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4293
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Fixing Attached Cells in Paraformaldehyde
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4294
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Fixing Suspension Cells with Paraformaldehyde
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4295
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Indirect Detection Using Horseradish Peroxidase-Labeled Reagents
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4296
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Indirect Detection Using Fluorochrome-Labeled Reagents
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4297
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Immunoblotting: Preparing Protein Solutions
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4298
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Immunoblotting: Preparing Cell Lysates
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4299
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Immunoblotting: Preparing Immunoprecipitated Proteins
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4300
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Genotyping Embryonic Stem (ES) Cell Colonies Prior to Picking
Philippe Soriano
CSH Protocols; 2006; doi:10.1101/pdb.prot4415
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Preparation of Embryonic Stem (ES) Cells for Injection
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4416
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Breaking the Tips of Embryonic Stem (ES) Cell Injection Needles
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4417
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Injecting Blastocysts
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4418
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Preparing Embryonic Stem (ES) Cells for Aggregation
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4419
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Preparing the Aggregation Plate
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4420
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Removal of Zona Pellucida
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4421
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Production of Tetraploid Embryos
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4422
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Assembling Aggregates between Diploid Embryos
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4423
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Assembling Aggregates between Embryonic Stem (ES) Cells and Diploid Embryos
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4424
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Assembling Aggregates between Diploid and Tetraploid Embryos
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4425
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Assembling Aggregates between Embryonic Stem (ES) Cells and Tetraploid Embryos
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4426
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Disaggregating Cleavage-Stage Embryos and the Inner Cell Mass (ICM) of Blastocysts into Individual Cells
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4427
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Immunosurgery: Isolating the Inner Cell Mass (ICM) of Blastocysts
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4428
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Growth and Concentration of Chlorogonium for Culturing Freshwater Hypotrichs
Carolyn Jahn
CSH Protocols; 2006; doi:10.1101/pdb.prot4464
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Flow Cytometric Analysis of Mitochondrial Transmembrane Potential (
m)
Shailaja Kasibhatla, Gustavo P. Amarante-Mendes, Deborah Finucane, Thomas Brunner, Ella Bossy-Wetzel, and Douglas R. Green
CSH Protocols; 2006; doi:10.1101/pdb.prot4465
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Mass Culture of Freshwater Hypotrichs
Carolyn Jahn
CSH Protocols; 2006; doi:10.1101/pdb.prot4466
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Culturing Marine Euplotids Using Dunaliella as a Food Source
Carolyn Jahn
CSH Protocols; 2006; doi:10.1101/pdb.prot4467
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Refeeding Marine Euplotids with Bacteria
Carolyn Jahn
CSH Protocols; 2006; doi:10.1101/pdb.prot4468
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Maintenance of Stocks of Marine Hypotrichs
Carolyn Jahn
CSH Protocols; 2006; doi:10.1101/pdb.prot4469
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Short-Term Storage of Tetrahymena Cultures
Melody T. Sweet and C. David Allis
CSH Protocols; 2006; doi:10.1101/pdb.prot4470
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Long-Term Storage Storage of Unfrozen Tetrahymena Cultures in Maintenance Culture Medium
Melody T. Sweet and C. David Allis
CSH Protocols; 2006; doi:10.1101/pdb.prot4471
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Long-Term Storage of Unfrozen Tetrahymena Cultures in Soybean Medium
Melody T. Sweet and C. David Allis
CSH Protocols; 2006; doi:10.1101/pdb.prot4472
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Leukostat Staining of Cytospin Preparations to Detect Apoptosis
Shailaja Kasibhatla, Gustavo P. Amarante-Mendes, Deborah Finucane, Thomas Brunner, Ella Bossy-Wetzel, and Douglas R. Green
CSH Protocols; 2006; doi:10.1101/pdb.prot4491
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Staining of Suspension Cells with Hoechst 33258 to Detect Apoptosis
Shailaja Kasibhatla, Gustavo P. Amarante-Mendes, Deborah Finucane, Thomas Brunner, Ella Bossy-Wetzel, and Douglas R. Green
CSH Protocols; 2006; doi:10.1101/pdb.prot4492
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Acridine Orange/Ethidium Bromide (AO/EB) Staining to Detect Apoptosis
Shailaja Kasibhatla, Gustavo P. Amarante-Mendes, Deborah Finucane, Thomas Brunner, Ella Bossy-Wetzel, and Douglas R. Green
CSH Protocols; 2006; doi:10.1101/pdb.prot4493
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Detection of Phosphatidylserine Externalization During Apoptosis
Shailaja Kasibhatla, Gustavo P. Amarante-Mendes, Deborah Finucane, Thomas Brunner, Ella Bossy-Wetzel, and Douglas R. Green
CSH Protocols; 2006; doi:10.1101/pdb.prot4494
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Preparation of dsRNA Molecules for RNAi in Mouse Oocytes and Early Embryos
Paula Stein and Petr Svoboda
CSH Protocols; 2006; doi:10.1101/pdb.prot4508
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Collection of Mouse Oocytes for RNAi
Paula Stein and Petr Svoboda
CSH Protocols; 2006; doi:10.1101/pdb.prot4509
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Collection of Early Mouse Embryos for RNAi
Paula Stein and Petr Svoboda
CSH Protocols; 2006; doi:10.1101/pdb.prot4510
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Microinjection of dsRNA into Mouse Oocytes and Early Embryos
Paula Stein and Petr Svoboda
CSH Protocols; 2006; doi:10.1101/pdb.prot4511
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Synthesis of dsRNA for RNAi in Drosophila: Plasmid Template Method
Richard W. Carthew
CSH Protocols; 2006; doi:10.1101/pdb.prot4512
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RNA Isolation and RT-PCR from dsRNA-Treated Mouse Oocytes and Early Embryos
Paula Stein and Petr Svoboda
CSH Protocols; 2006; doi:10.1101/pdb.prot4513
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Synthesis of dsRNA for RNAi in Drosophila: PCR Template Method
Richard W. Carthew
CSH Protocols; 2006; doi:10.1101/pdb.prot4514
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Synthesis of siRNA for RNAi in Drosophila
Richard W. Carthew
CSH Protocols; 2006; doi:10.1101/pdb.prot4515
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Microinjection of dsRNA into Drosophila Embryos
Richard W. Carthew
CSH Protocols; 2006; doi:10.1101/pdb.prot4516
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Delivery of dsRNA into Drosophila Embryos by Gene Gun
Richard W. Carthew
CSH Protocols; 2006; doi:10.1101/pdb.prot4517
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Semiquantitative RT-PCR for Phenotype Analysis of RNAi-Treated Drosophila Embryos
Richard W. Carthew
CSH Protocols; 2006; doi:10.1101/pdb.prot4518
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Transcript In Situ Hybridization of Whole-Mount Embryos for Phenotype Analysis of RNAi-Treated Drosophila
Richard W. Carthew
CSH Protocols; 2006; doi:10.1101/pdb.prot4519
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