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Cover Illustration: Acceptor photobleaching experiment on fixed MCF7 cells detecting the phosphorylation state of ErbB1-GFP receptors using an anti-phosphotyrosine antibody labeled with Cy3. Image from Imaging Protein Interactions by FRET Microscopy: FRET Measurements by Acceptor Photobleaching (doi:10.1101/pdb.prot4598).
G1 Synchronization of CHO Cells by Isoleucine Deprivation
Paul J. Galgano and Carl L. Schildkraut
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4486
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G1/S Phase Synchronization Using Mimosine Arrest
Paul J. Galgano and Carl L. Schildkraut
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4488
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Synchronization of Mammalian Cell Cultures in Mitosis Using Selective Detachment
Paul J. Galgano and Carl L. Schildkraut
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4489
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Freezing and Thawing Tetrahymena Cultures
Melody T. Sweet and C. David Allis
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4497
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Vegetative Growth of Tetrahymena
Melody T. Sweet and C. David Allis
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4498
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Induction of Conjugation in Tetrahymena
Melody T. Sweet and C. David Allis
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4499
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Isolation and Purification of Tetrahymena Nuclei
Melody T. Sweet and C. David Allis
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4500
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Transformation of Tetrahymena thermophila by Electroporation
Melody T. Sweet and C. David Allis
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4502
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Production of dsRNA for RNAi in Avian Embryos
Esther T. Stoeckli
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4503
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Injection of dsRNA and Electroporation in Avian Embryos
Esther T. Stoeckli
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4504
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Dissection of Spinal Cords for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos
Esther T. Stoeckli
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4505
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Whole-Mount Preparations for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos
Esther T. Stoeckli
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4506
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Staining of Tissue Slices for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos
Esther T. Stoeckli
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4507
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Staining Yeast
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4520
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Fixing Caenorhabditis elegans in Bouin's fixative
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4521
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Fixing Caenorhabditis elegans in Paraformaldehyde
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4522
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Antibody Addition and Detection for Staining Caenorhabditis elegans
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4523
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Preparing Early Whole-Mount Drosophila Embryos for Immunostaining
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4524
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Preparing Late Whole-Mount Drosophila Embryos for Immunostaining
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4525
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Antibody Addition to Drosophila Specimens and Detection Using Enzyme-Linked Reagents
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4526
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Antibody Addition to Drosophila Specimens and Detection Using Fluorochrome-Linked Reagents
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4527
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Unmasking Hidden Epitopes with Proteases
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4528
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Unmasking Hidden Epitopes Using the Microwave Oven
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4529
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Unmasking Hidden Epitopes Using the Pressure Cooker
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4530
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Lysing Tissue-Culture Cells for Immunoprecipitation
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4531
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Immunoprecipitation: Lysing Bacteria by Sonication
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4532
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Immunoprecipitation: Lysing Yeast Cells Using Glass Beads
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4533
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Immunoprecipitation: Denaturing Lysis
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4534
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Immunoprecipitation: Preclearing the Lysate
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4535
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Immunoprecipitation: Purifying the Immune Complexes
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4536
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Fragmentation of DNA by Sonication
Joseph Sambrook and David W. Russell
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4538
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Fragmentation of DNA by Nebulization
Joseph Sambrook and David W. Russell
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4539
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SDS-Polyacrylamide Gel Electrophoresis of Proteins
Joseph Sambrook and David W. Russell
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4540
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Staining SDS-Polyacrylamide Gels with Silver Salts
Joseph Sambrook and David W. Russell
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4541
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Drying SDS-Polyacrylamide Gels
Joseph Sambrook and David W. Russell
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4542
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Chromatin Immunoprecipitation (ChIP) of Protein Complexes: Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells
Achim Breiling and Valerio Orlando
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4560
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Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array: Amplification of ORFs
Tony R. Hazbun and John P. Miller
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4561
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Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array: Cloning by Homologous Recombination and High-Throughput Transformation
Tony R. Hazbun and John P. Miller
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4562
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Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array: High-Density Robotic Screening Procedure
Tony R. Hazbun and John P. Miller
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4563
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Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array: Storage, Maintenance, and Working with Living Arrays
Tony R. Hazbun and John P. Miller
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4564
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Analysis of Protein Interactions with Immobilized Peptide Arrays Synthesized on Membrane Supports
Ronald Frank and Stefan Dubel
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4566
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Troubleshooting and Limitations of the Tandem Affinity Purification
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip7
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