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Cover Illustration: Movable Plexiglas stage containing nano-LC electrospray power source. Image from Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT) (doi:10.1101/pdb.prot4555).
Staining Membrane-Bound Proteins with Ponceau S
Wayne R. Stochaj, Tom Berkelman, and Nancy Laird
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4543
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Staining Membrane-Bound Proteins with Coomassie Blue R250
Wayne R. Stochaj, Tom Berkelman, and Nancy Laird
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4544
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Staining Membrane-Bound Proteins with Colloidal Gold
Wayne R. Stochaj, Tom Berkelman, and Nancy Laird
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4545
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Packing Capillary Columns for RP-HPLC
Robert Moritz
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4546
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Purification of Large "Troublesome" Polypeptides by RP-HPLC
Hooi Hong Keah and Milton T.W. Hearn
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4547
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Purification of Peptides from Solid-Phase Peptide Synthesis with RP-HPLC
Reinhard I. Boysen and Milton T.W. Hearn
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4548
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Desalting of Peptides and Protein Mixtures by RP-HPLC Techniques
Reinhard I. Boysen and Milton T.W. Hearn
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4549
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Fragmentation of Protein Using Trypsin
Richard J. Simpson
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4550
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Modification of Lysyl Side Chains Using Succinic Anhydride
Richard J. Simpson
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4551
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Modification of Lysyl Side Chains Using Citraconic Anhydride
Richard J. Simpson
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4552
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Analysis of Complex Protein Mixtures Using Nano-LC Coupled to MS/MS
David M. Schieltz, Michael P. Washburn, and Lara G. Hays
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4553
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Tandem Mass Spectrometry Analysis Using the ThermoFinnigan LCQ System
David M. Schieltz, Michael P. Washburn, and Lara G. Hays
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4554
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Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT)
David M. Schieltz and Michael P. Washburn
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4555
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Digestion of Insoluble Protein Fractions for Multidimensional Protein Identification Technology (MuDPIT) Analysis
David M. Schieltz and Michael P. Washburn
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4556
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Using FLAG Epitope-Tagged Proteins for Coimmunoprecipitation of Interacting Proteins
Anne Verhagen
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4557
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Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: SDS-PAGE of Proteins
Wayne R. Stochaj, Tom Berkelman, and Nancy Laird
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4559
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Fluorescent Staining of Proteins with SYPRO Ruby
Richard J. Simpson
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4567
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Preparation of Eukaryotic Lysates for 2D Gel Electrophoresis
Hong Ji
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4571
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Radioisotopic Labeling of Eukaryotic Cell Proteins Using 35S
Hong Ji
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4572
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Radioisotopic Labeling of Eukaryotic Cell Proteins Using 32P
Hong Ji
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4573
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Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: Rehydration of IPG Strips for Isoelectric Focusing of Proteins
Wayne R. Stochaj, Tom Berkelman, and Nancy Laird
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4574
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Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: Isoelectric Focusing of Proteins in a Multipurpose Flatbed Electrophoresis Unit
Wayne R. Stochaj, Tom Berkelman, and Nancy Laird
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4575
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Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: IEF of Proteins in an IEF-Dedicated Electrophoresis Unit
Wayne R. Stochaj, Tom Berkelman, and Nancy Laird
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4576
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Phosphoprotein Staining with the GelCode Phosphoprotein Staining Kit
Wayne R. Stochaj, Tom Berkelman, and Nancy Laird
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4577
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Analysis of Protein Interactions In Vivo with Fluorescence Resonance Energy Transfer (FRET)
Marc Damelin and Pamela Silver
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4581
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Preparation of Vertical SDS Slab Gels: Casting a Single Homogeneous Gel
Wayne R. Stochaj, Tom Berkelman, and Nancy Laird
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4582
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Preparation of Vertical SDS Slab Gels: Simultaneous Casting of Multiple-Gradient Gels
Wayne R. Stochaj, Tom Berkelman, and Nancy Laird
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4583
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Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: IPG Strip Equilibration
Wayne R. Stochaj, Tom Berkelman, and Nancy Laird
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4584
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Concentrating Acrylamide Gel Spots
Richard J. Simpson
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4585
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Gateway-Compatible Yeast One-Hybrid Screens
Bart Deplancke, Vanessa Vermeirssen, H. Efsun Arda, Natalia J. Martinez, and Albertha J.M. Walhout
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4590
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EMS Mutagenesis of Arabidopsis Seed
Detlef Weigel and Jane Glazebrook
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4621
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Screening DNA Pools for T-DNA Insertions in Arabidopsis Genes
Detlef Weigel and Jane Glazebrook
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4622
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Setting Up Arabidopsis Crosses
Detlef Weigel and Jane Glazebrook
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4623
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Chromatographic Conditions for the Analytical Separation of Crude Peptide and Protein Mixtures
Reinhard I. Boysen and Milton T.W. Hearn
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip3
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Chromatographic Conditions for the Preparative Separation of Peptides
Reinhard I. Boysen and Milton T.W. Hearn
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip4
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The Use of Mass Spectrometry in Proteomics: Database Searching
David M. Schieltz, Michael P. Washburn, and Lara G. Hays
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip5
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Chromatographic Conditions for the Desalting of Peptides and Proteins
Reinhard I. Boysen and Milton T.W. Hearn
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip6
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Equipment for In Vivo FRET Analysis
Marc Damelin and Pamela Silver
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip8
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An Explanation of Symbols Used for Analysis of FRET Data
Marc Damelin and Pamela Silver
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip9
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Experimental Design for In Vivo FRET Analysis
Marc Damelin and Pamela Silver
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip10
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Cultivation of Arabidopsis
Daphne Preuss
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip22
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Forward Genetics in Arabidopsis: Finding Mutations that Cause Particular Phenotypes
Detlef Weigel and Jane Glazebrook
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.top1
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