Cold Spring Harbor Protocols, Table of Contents
Volume 2009, Issue 10

October 2009

Cover Illustration: Fluorescence 3D reconstruction of the Nkx2-5^+/GFP mouse embryonic heart. GFP was targeted into the Nkx2-5 locus resulting in GFP expression specifically in cardiomyocytes (Biben C, Weber R, Kesteven S, Stanley E, McDonald L, Elliott DA, Barnett L, Köentgen F, Robb L, Feneley M, et al. 2000. Cardian septal and valvular dysmorphogenesis in mice heterozygous for mutations in the homeobox gene Nkx2-5. Circ Res 87: 888-895). Embryos were harvested at E9.5; heart tissue was dissected and fixed in 4% paraformaldehyde. A z-stack was acquired with a Zeiss LSM510 META confocal microscope using a 20X objective and a reconstructed 3D projection was generated using the LSM software. (Image courtesy of Aya Wada and Mary Dickinson.) For information on processing GFP-expressing mouse embryos, see Fixation and Paraffin-Embedding of Mouse Tissues for GFP Visualization (doi:10.1101/pdb.prot5298).

Emerging Model Organisms

Maize (Zea mays): A Model Organism for Basic and Applied Research in Plant Biology
Josh Strable and Michael J. Scanlon
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.emo132
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The ‘Mother of Thousands’ (Kalanchoë daigremontiana): A Plant Model for Asexual Reproduction and CAM Studies
Helena Garcês and Neelima Sinha
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.emo133
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The Parasitoid Wasp Nasonia: An Emerging Model System with Haploid Male Genetics
John H. Werren and David W. Loehlin
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.emo134
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Topic Introduction

High-Resolution Dual-Trap Optical Tweezers with Differential Detection: An Introduction
Carlos Bustamante, Yann R. Chemla, and Jeffrey R. Moffitt
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.top60
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Information Panels

Haploview: Visualization and Analysis of SNP Genotype Data
Jeffrey C. Barrett
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.ip71
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High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Managing Environmental Noise
Carlos Bustamante, Yann R. Chemla, and Jeffrey R. Moffitt
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.ip72
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High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Instrument Design
Carlos Bustamante, Yann R. Chemla, and Jeffrey R. Moffitt
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.ip73
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High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Data Collection and Instrument Calibration
Carlos Bustamante, Yann R. Chemla, and Jeffrey R. Moffitt
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.ip74
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High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Minimizing the Influence of Measurement Noise
Carlos Bustamante, Yann R. Chemla, and Jeffrey R. Moffitt
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.ip75
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High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Alignment of Instrument Components
Carlos Bustamante, Yann R. Chemla, and Jeffrey R. Moffitt
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.ip76
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Protocols

Phenotypic Analysis of Arabidopsis Mutants: Oomycete Pathogens
Joseph D. Clarke
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot4984
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Fixation and Paraffin-Embedding of Mouse Tissues for GFP Visualization
Hugo Vankelecom
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5298
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Analysis of In Vivo Protein Complexes by Coimmunoprecipitation from Caenorhabditis elegans
Britta Jedamzik and Christian R. Eckmann
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5299
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Analysis of RNA-Protein Complexes by RNA Coimmunoprecipitation and RT-PCR Analysis from Caenorhabditis elegans
Britta Jedamzik and Christian R. Eckmann
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5300
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Fixing and Sectioning Tissue from the Plant Kalanchoë daigremontiana
Helena Garcês and Neelima Sinha
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5301
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In Situ Hybridization in the Plant Kalanchoë daigremontiana
Helena Garcês and Neelima Sinha
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5302
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Transformation of the Plant Kalanchoë daigremontiana using Agrobacterium tumefaciens
Helena Garcês and Neelima Sinha
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5303
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Extraction of DNA from the Plant Kalanchoë daigremontiana
Helena Garcês and Neelima Sinha
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5304
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Extraction of RNA from the Plant Kalanchoë daigremontiana
Helena Garcês and Neelima Sinha
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5305
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Formation and Use of Water-Soluble Lipopolymer-pDNA and Lipopeptide-pDNA Complexes
Ram I. Mahato, Zhaoyang Ye, and Sung Wan Kim
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5306
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Strain Maintenance of Nasonia vitripennis (Parasitoid Wasp)
John H. Werren and David W. Loehlin
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5307
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Rearing Sarcophaga bullata Fly Hosts for Nasonia (Parasitoid Wasp)
John H. Werren and David W. Loehlin
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5308
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Egg Collection for Nasonia (Parasitoid Wasp)
John H. Werren and David W. Loehlin
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5309
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Virgin Collection and Haplodiploid Crossing Methods in Nasonia (Parasitoid Wasp)
John H. Werren and David W. Loehlin
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5310
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Larval RNA in Nasonia (Parasitoid Wasp)
John H. Werren, David W. Loehlin, and Jonathan D. Giebel
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5311
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Curing Wolbachia Infections in Nasonia (Parasitoid Wasp)
John H. Werren and David W. Loehlin
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5312
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Field Collection of Nasonia (Parasitoid Wasp) Using Baits
John H. Werren and David W. Loehlin
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5313
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