Cold Spring Harbor Protocols, Table of Contents
Volume 2009, Issue 11

November 2009

Cover Illustration: Image of two juveniles of the Hawaiian bobtail squid Euprymna scolopes (top, ~1.5 mm in length) and an adult individual (lower panel, ~4 cm in length). The ink sac and funnel tube are visible on the posterior side (right) of the recently hatched animals. The larger fourth arm pair is a tentacle specialized for prey capture. (Image courtesy of Patricia Lee.) For more information on the embryonic development of squid embryos, see The Hawaiian Bobtail Squid (Euprymna scolopes): A Model to Study the Molecular Basis of Eukaryote-Prokaryote Mutualism and the Development and Evolution of Morphological Novelties in Cephalopods (doi:10.1101/pdb.emo135).

Emerging Model Organisms

The Hawaiian Bobtail Squid (Euprymna scolopes): A Model to Study the Molecular Basis of Eukaryote-Prokaryote Mutualism and the Development and Evolution of Morphological Novelties in Cephalopods
Patricia N. Lee, Margaret J. McFall-Ngai, Patrick Callaerts, and H. Gert de Couet
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.emo135
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True Yams (Dioscorea): A Biological and Evolutionary Link between Eudicots and Grasses
Hodeba D. Mignouna, Mathew M. Abang, Robert Asiedu, and R. Geeta
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.emo136
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Topic Introduction

Assessing Human Variation Data for Signatures of Natural Selection
Mike Bamshad and J. Claiborne Stephens
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.top61
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Laboratory Methods for High-Throughput Genotyping
Howard J. Edenberg and Yunlong Liu
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.top62
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Information Panels

The Embryonic Development of the Hawaiian Bobtail Squid (Euprymna scolopes)
Patricia N. Lee, Patrick Callaerts, and H. Gert de Couet
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.ip77
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Protocols

Labeling Pinocytotic Vesicles and Cytoplasm with Fluorescent Dextrans or Ficolls for Imaging
Brad Chazotte
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot4951
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Phenotypic Analysis of Arabidopsis Mutants: Root Elongation under Salt/Hormone-Induced Stress
Byeong-ha Lee and Jian-Kang Zhu
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot4968
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Preparation of Soluble GST Fusion Proteins
Ilaria Rebay and Richard G. Fehon
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot4996
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Preparation of Insoluble GST Fusion Proteins
Ilaria Rebay and Richard G. Fehon
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot4997
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Imaging Synaptogenesis by Measuring Accumulation of Synaptic Proteins
Camin Dean and Peter Scheiffele
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5315
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Imaging Action Potentials with Calcium Indicators
Jason N. MacLean and Rafael Yuste
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5316
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Indirect Immunofluorescence Labeling in the Yeast Saccharomyces cerevisiae
Pamela Silver
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5317
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Bimolecular Affinity Purification (BAP): Tandem Affinity Purification Using Two Protein Baits
Gabriel N. Maine, Nathan Gluck, Iram W. Zaidi, and Ezra Burstein
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5318
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Preparation of Genomic DNA from Hawaiian Bobtail Squid (Euprymna scolopes) Tissue by Cesium Chloride Gradient Centrifugation
Patricia N. Lee, Margaret J. McFall-Ngai, Patrick Callaerts, and H. Gert de Couet
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5319
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Confocal Immunocytochemistry of Embryonic and Juvenile Hawaiian Bobtail Squid (Euprymna scolopes) Tissues
Patricia N. Lee, Margaret J. McFall-Ngai, Patrick Callaerts, and H. Gert de Couet
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5320
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Whole-Mount In Situ Hybridization of Hawaiian Bobtail Squid (Euprymna scolopes) Embryos with DIG-Labeled Riboprobes: I. DNA Template Preparation and In Vitro Transcription of Riboprobes
Patricia N. Lee, Margaret J. McFall-Ngai, Patrick Callaerts, and H. Gert de Couet
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5321
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Whole-Mount In Situ Hybridization of Hawaiian Bobtail Squid (Euprymna scolopes) Embryos with DIG-Labeled Riboprobes: II. Embryo Preparation, Hybridization, Washes, and Immunohistochemistry
Patricia N. Lee, Margaret J. McFall-Ngai, Patrick Callaerts, and H. Gert de Couet
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5322
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Culture of Hawaiian Bobtail Squid (Euprymna scolopes) Embryos and Observation of Normal Development
Patricia N. Lee, Patrick Callaerts, and H. Gert de Couet
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5323
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Yam (Dioscorea) Husbandry: Cultivating Yams in the Field or Greenhouse
Hodeba D. Mignouna, Mathew M. Abang, Robert Asiedu, and R. Geeta
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5324
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Culturing Meristematic Tissue and Node Cuttings from Yams (Dioscorea)
Hodeba D. Mignouna, Mathew M. Abang, Robert Asiedu, and R. Geeta
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5325
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Post-Flask Management of Yam (Dioscorea) Plantlets
Hodeba D. Mignouna, Mathew M. Abang, Robert Asiedu, and R. Geeta
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5326
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Producing Yam (Dioscorea) Seeds through Controlled Crosses
Hodeba D. Mignouna, Mathew M. Abang, Robert Asiedu, and R. Geeta
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5327
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Extraction of DNA from Yam (Dioscorea) Leaves
Hodeba D. Mignouna, Mathew M. Abang, Robert Asiedu, and R. Geeta
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5328
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