Cold Spring Harbor Protocols, Table of Contents
Volume 2006, Issue 7

December 2006

Cover Illustration: Overview of the Affinity Purification - Mass Spectrometry procedure. Image from Overview of Affinity Purification in Combination with Mass Spectrometry (doi:10.1101/pdb.top3).

Protocols

6-Azauracil Sensitivity Assay for Yeast
William P. Tansey
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4613
Abstract
Full Text

Freeze-Thaw Lysis for Extraction of Proteins from Mammalian Cells
William P. Tansey
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4614
Abstract
Full Text

Detection of Ubiquitylated Proteins in Yeast
William P. Tansey
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4615
Abstract
Full Text

Constructing and Expressing GFP Fusion Proteins
David L. Spector and Robert D. Goldman
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4649
Abstract
Full Text

Plating Cells for Microinjection
David A. Dean
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4650
Abstract
Full Text

Preparation (Pulling) of Needles for Gene Delivery by Microinjection
David A. Dean
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4651
Abstract
Full Text

DNA Preparation and Needle Loading for Gene Delivery by Microinjection
David A. Dean
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4652
Abstract
Full Text

Gene Delivery by Direct Injection (Microinjection) Using a Pulsed-Flow System
David A. Dean
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4653
Abstract
Full Text

Gene Delivery by Direct Injection (Microinjection) Using a Controlled-Flow System
David A. Dean
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4654
Abstract
Full Text

Silver Staining, Digestion, and Extraction of Peptides from an Acrylamide Gel for MS Analysis
Sherry Niessen, Ian Mcleod, and John R. Yates, III
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4661
Abstract
Full Text

Direct Enzymatic Digestion of Protein Complexes for MS Analysis
Sherry Niessen, Ian Mcleod, and John R. Yates, III
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4662
Abstract
Full Text

HPLC Separation of Digested Proteins and Preparation for Matrix-Assisted Laser Desorption/Ionization Analysis
Sherry Niessen, Ian Mcleod, and John R. Yates, III
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4663
Abstract
Full Text

Transformation of Agrobacterium Using Electroporation
Detlef Weigel and Jane Glazebrook
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4665
Abstract
Full Text

Transformation of Agrobacterium Using the Freeze-Thaw Method
Detlef Weigel and Jane Glazebrook
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4666
Abstract
Full Text

PCR Analysis of Agrobacterium
Phil Wigge
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4667
Abstract
Full Text

In Planta Transformation of Arabidopsis
Detlef Weigel and Jane Glazebrook
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4668
Abstract
Full Text

Kanamycin Selection of Transformed Arabidopsis
Detlef Weigel and Jane Glazebrook
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4669
Abstract
Full Text

Glufosinate Ammonium Selection of Transformed Arabidopsis
Detlef Weigel and Jane Glazebrook
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4670
Abstract
Full Text

Root Transformation of Arabidopsis
Detlef Weigel and Jane Glazebrook
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4671
Abstract
Full Text

Information Panel

Vectors and Agrobacterium Hosts for Arabidopsis Transformation
Detlef Weigel and Jane Glazebrook
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip29
Abstract
Full Text

Topic Introduction

Overview of Affinity Purification in Combination with Mass Spectrometry
Sherry Niessen, Ian Mcleod, and John R. Yates, III
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.top3
Abstract
Full Text