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Cover Illustration: Fluorescence image of a GFP-transfected cerebellar granule neuron overlaid with Hoescht staining to identify nuclei of all cells in culture. The cells were transfected on DIV1 and fixed on DIV5, then subject to immunostaining for GFP (labeled with a Cy3-conjugated secondary). (Image courtesy of Parizad M. Bilimoria and Azad Bonni.) For more information on this culture technique, see Cultures of Cerebellar Granule Neurons (doi:10.1101/pdb.prot5107).
The Demosponge Amphimedon queenslandica: Reconstructing the Ancestral Metazoan Genome and Deciphering the Origin of Animal Multicellularity
Bernard M. Degnan, Maja Adamska, Alina Craigie, Sandie M. Degnan, Bryony Fahey, Marie Gauthier, John N.A. Hooper, Claire Larroux, Sally P. Leys, Erica Lovas, and Gemma S. Richards
CSH Protocols; 2008; doi:10.1101/pdb.emo108
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Dictyostelium discoideum: The Social Ameba
Pascale Gaudet, Petra Fey, and Rex Chisholm
CSH Protocols; 2008; doi:10.1101/pdb.emo109
Abstract
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The Two-Spotted Cricket Gryllus bimaculatus: An Emerging Model for Developmental and Regeneration Studies
Taro Mito and Sumihare Noji
CSH Protocols; 2008; doi:10.1101/pdb.emo110
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The Dogfish Scyliorhinus canicula: A Reference in Jawed Vertebrates
Marion Coolen, Arnaud Menuet, Danièle Chassoux, Claudia Compagnucci, Sébastien Henry, Laurent Lévèque, Corinne Da Silva, Frédérick Gavory, Sylvie Samain, Patrick Wincker, Claude Thermes, Yves D’Aubenton-Carafa, Isabel Rodriguez-Moldes, Gavin Naylor, Michael Depew, Pascal Sourdaine, and Sylvie Mazan
CSH Protocols; 2008; doi:10.1101/pdb.emo111
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Using the Nucleic Acid Programmable Protein Array (NAPPA) for Identifying Protein-Protein Interactions: General Guidelines
Andrew J. Link and Joshua LaBaer
CSH Protocols; 2008; doi:10.1101/pdb.ip62
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Bone Marrow-Derived Macrophages (BMM): Isolation and Applications
Joachim Weischenfeldt and Bo Porse
CSH Protocols; 2008; doi:10.1101/pdb.prot5080
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Gene Silencing by Injection and Electroporation of dsRNA in Avian Embryos
Olivier Mauti, Thomas Baeriswyl, and Esther T. Stoeckli
CSH Protocols; 2008; doi:10.1101/pdb.prot5094
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Isolation of Amphimedon Developmental Material
Sally P. Leys, Claire Larroux, Marie Gauthier, Maja Adamska, Bryony Fahey, Gemma S. Richards, Sandie M. Degnan, and Bernard M. Degnan
CSH Protocols; 2008; doi:10.1101/pdb.prot5095
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Whole-Mount In Situ Hybridization in Amphimedon
Claire Larroux, Bryony Fahey, Maja Adamska, Gemma S. Richards, Marie Gauthier, Kathryn Green, Erica Lovas, and Bernard M. Degnan
CSH Protocols; 2008; doi:10.1101/pdb.prot5096
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Analysis of Cell Movement in Amphimedon Embryos by Injection of Fluorescent Tracers
Maja Adamska and Bernard M. Degnan
CSH Protocols; 2008; doi:10.1101/pdb.prot5097
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Genotyping Individual Amphimedon Embryos, Larvae, and Adults
Sandie M. Degnan, Alina Craigie, and Bernard M. Degnan
CSH Protocols; 2008; doi:10.1101/pdb.prot5098
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Growth and Maintenance of Dictyostelium Cells
Pascale Gaudet, Petra Fey, and Rex Chisholm
CSH Protocols; 2008; doi:10.1101/pdb.prot5099
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Multicellular Development of Dictyostelium
Pascale Gaudet, Petra Fey, and Rex Chisholm
CSH Protocols; 2008; doi:10.1101/pdb.prot5100
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Making Permanent Stocks of Dictyostelium
Pascale Gaudet, Petra Fey, and Rex Chisholm
CSH Protocols; 2008; doi:10.1101/pdb.prot5101
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Transformation of Dictyostelium with Plasmid DNA by Calcium Phosphate Precipitation
Pascale Gaudet, Petra Fey, and Rex Chisholm
CSH Protocols; 2008; doi:10.1101/pdb.prot5102
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Transformation of Dictyostelium with Plasmid DNA by Electroporation
Pascale Gaudet, Petra Fey, and Rex Chisholm
CSH Protocols; 2008; doi:10.1101/pdb.prot5103
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Selection of Dictyostelium Transformants
Pascale Gaudet, Petra Fey, and Rex Chisholm
CSH Protocols; 2008; doi:10.1101/pdb.prot5104
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Extraction of DNA from Dictyostelium
Pascale Gaudet, Petra Fey, and Rex Chisholm
CSH Protocols; 2008; doi:10.1101/pdb.prot5105
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Extraction of RNA from Dictyostelium
Pascale Gaudet, Petra Fey, and Rex Chisholm
CSH Protocols; 2008; doi:10.1101/pdb.prot5106
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Cultures of Cerebellar Granule Neurons
Parizad M. Bilimoria and Azad Bonni
CSH Protocols; 2008; doi:10.1101/pdb.prot5107
Abstract
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Using the Nucleic Acid Programmable Protein Array (NAPPA) for Identifying Protein-Protein Interactions. Protocol 1: Coexpression of Query Protein on NAPPA Slides
Andrew J. Link and Joshua LaBaer
CSH Protocols; 2008; doi:10.1101/pdb.prot5108
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Using the Nucleic Acid Programmable Protein Array (NAPPA) for Identifying Protein-Protein Interactions. Protocol 2: Detection of Query Protein on NAPPA Slides
Andrew J. Link and Joshua LaBaer
CSH Protocols; 2008; doi:10.1101/pdb.prot5109
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Collection, Cryopreservation, Storage, and Revitalization of Transgenic Mouse Embryos
Anna Schwab and Johannes Schenkel
CSH Protocols; 2008; doi:10.1101/pdb.prot5111
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Chicken Erythrocyte Histone Octamer Preparation
Craig L. Peterson and Jeffrey C. Hansen
CSH Protocols; 2008; doi:10.1101/pdb.prot5112
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Salt Gradient Dialysis Reconstitution of Nucleosomes
Craig L. Peterson
CSH Protocols; 2008; doi:10.1101/pdb.prot5113
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