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Cover Illustration: Composite fluorescence image of cultured HeLa cells at different stages of the cell cycle. Cells are expressing a recombinant mannosidase II-EGFP targeted to the Golgi apparatus (green), then immunolabeled for tubulin (red) and counterstained for DNA (blue). Multiphoton microscopy using a BioRad RTS2000-MP imaging system on a Nikon TE300 inverted microscope and a Nikon 60X 1.45 objective lens. (Image courtesy of Thomas Deerinck and Mark Ellisman.) For more information on fluorescent proteins and their variants, see Fluorescent Protein Tracking and Detection: Fluorescent Protein Structure and Color Variants (doi:10.1101/pdb.top63).
The Tammar Wallaby, Macropus eugenii: A Model Kangaroo for the Study of Developmental and Reproductive Biology
Danielle Hickford, Stephen Frankenberg, and Marilyn B. Renfree
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.emo137
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The Sea Squirt Ciona intestinalis
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Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.emo138
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Fluorescent Protein Tracking and Detection: Fluorescent Protein Structure and Color Variants
Mark A. Rizzo, Michael W. Davidson, and David W. Piston
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.top63
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Fluorescent Protein Tracking and Detection: Applications Using Fluorescent Proteins in Living Cells
Mark A. Rizzo, Michael W. Davidson, and David W. Piston
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.top64
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Computational Image Analysis of Cellular Dynamics: A Case Study Based on Particle Tracking
Khuloud Jaqaman and Gaudenz Danuser
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.top65
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Genome-Wide Association Studies
Ammar Al-Chalabi
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.top66
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Dignam and Roeder Nuclear Extract Preparation
Michael F. Carey, Craig L. Peterson, and Stephen T. Smale
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5330
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In Vitro Transcription Using HeLa Cell Extracts and Primer Extension
Michael F. Carey, Craig L. Peterson, and Stephen T. Smale
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5331
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Working with Tammar Wallabies (Macropus eugenii)
Danielle Hickford, Stephen Frankenberg, and Marilyn B. Renfree
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5332
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Performing Surgery on Tammar Wallaby (Macropus eugenii) Adults
Danielle Hickford, Stephen Frankenberg, and Marilyn B. Renfree
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5333
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Surgery on Tammar Wallaby (Macropus eugenii) Pouch Young
Danielle Hickford, Stephen Frankenberg, and Marilyn B. Renfree
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5334
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Collection, Handling, Fixation, and Processing of Tammar Wallaby (Macropus eugenii) Embryos
Danielle Hickford, Stephen Frankenberg, and Marilyn B. Renfree
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5335
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Culturing Tammar Wallaby (Macropus eugenii) Pouch Young Gonads
Danielle Hickford, Stephen Frankenberg, and Marilyn B. Renfree
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5336
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Culturing Tammar Wallaby (Macropus eugenii) Peri-gastrulation Stage Embryos
Danielle Hickford, Stephen Frankenberg, and Marilyn B. Renfree
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5337
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Immunohistochemical Staining of Sectioned Tammar Wallaby (Macropus eugenii) Tissue
Danielle Hickford, Stephen Frankenberg, and Marilyn B. Renfree
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5338
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Whole-Mount Immunohistochemical Staining of Tammar Wallaby (Macropus eugenii) Cleavage Stages and Blastocysts
Danielle Hickford, Stephen Frankenberg, and Marilyn B. Renfree
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5339
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Whole-Mount Immunohistochemical Staining of Tammar Wallaby (Macropus eugenii) Peri-gastrulation Embryos
Danielle Hickford, Stephen Frankenberg, and Marilyn B. Renfree
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5340
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Analyzing Microorganisms in Environmental Samples Using Stable Isotope Probing with H218O
Egbert Schwartz
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5341
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Amplification of Bisulfite-Converted DNA for Genome-Wide DNA Methylation Profiling
Jon Reinders
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5342
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Biarsenical Labeling of Tetracysteine-Tagged Proteins for Tracking Existing and Newly Synthesized Pools of Proteins
Asit K. Pattnaik and Debasis Panda
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5343
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Isolation of Sea Squirt (Ciona) Gametes, Fertilization, Dechorionation, and Development
Lionel Christiaen, Eileen Wagner, Weiyang Shi, and Michael Levine
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5344
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Electroporation of Transgenic DNAs in the Sea Squirt (Ciona)
Lionel Christiaen, Eileen Wagner, Weiyang Shi, and Michael Levine
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5345
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X-gal Staining of Electroporated Sea Squirt (Ciona) Embryos
Lionel Christiaen, Eileen Wagner, Weiyang Shi, and Michael Levine
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5346
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Microinjection of Morpholino Oligos and RNAs in Sea Squirt (Ciona) Embryos
Lionel Christiaen, Eileen Wagner, Weiyang Shi, and Michael Levine
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5347
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Whole-Mount In Situ Hybridization on Sea Squirt (Ciona) Embryos
Lionel Christiaen, Eileen Wagner, Weiyang Shi, and Michael Levine
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5348
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Isolation of Individual Cells and Tissues from Electroporated Sea Squirt (Ciona) Embryos by Fluorescence-Activated Cell Sorting (FACS)
Lionel Christiaen, Eileen Wagner, Weiyang Shi, and Michael Levine
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5349
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