Cold Spring Harbor Protocols, Table of Contents
Volume 4, Issue 1

January 2009

Cover Illustration: Transverse section of Syn.H2B-eGFP transgenic quail showing optic tectum and cerebellum at day 1 post-hatch. The section is counterstained with DAPI. (Image courtesy of David Huss and Rusty Lansford.) For more information on the methods used, see Japanese Quail: An Efficient Animal Model for the Production of Transgenic Avians (doi:10.1101/pdb.emo112).

Emerging Model Organisms

Japanese Quail: An Efficient Animal Model for the Production of Transgenic Avians
Greg Poynter, David Huss, and Rusty Lansford
CSH Protocols; 2009; doi:10.1101/pdb.emo112
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The Sea Lamprey Petromyzon marinus: A Model for Evolutionary and Developmental Biology
Natalya Nikitina, Marianne Bronner-Fraser, and Tatjana Sauka-Spengler
CSH Protocols; 2009; doi:10.1101/pdb.emo113
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The Crustacean Parhyale hawaiensis: A New Model for Arthropod Development
E. Jay Rehm, Roberta L. Hannibal, R. Crystal Chaw, Mario A. Vargas-Vila, and Nipam H. Patel
CSH Protocols; 2009; doi:10.1101/pdb.emo114
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Topic Introductions

RNAi Experiments in Mouse Oocytes and Early Embryos
Petr Svoboda and Paula Stein
CSH Protocols; 2009; doi:10.1101/pdb.top56
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Information Panels

Choosing the Sequence of dsRNA for RNAi in Mouse
Petr Svoboda
CSH Protocols; 2009; doi:10.1101/pdb.ip63
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Cloning and Sequencing an Inverted Repeat
Petr Svoboda
CSH Protocols; 2009; doi:10.1101/pdb.ip64
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Protocols

Generation of High-Titer Lentivirus for the Production of Transgenic Quail
Greg Poynter, David Huss, and Rusty Lansford
CSH Protocols; 2009; doi:10.1101/pdb.prot5117
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Injection of Lentivirus into Stage-X Blastoderm for the Production of Transgenic Quail
Greg Poynter, David Huss, and Rusty Lansford
CSH Protocols; 2009; doi:10.1101/pdb.prot5118
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Screening for Transgenic Japanese Quail Offspring
Greg Poynter, David Huss, and Rusty Lansford
CSH Protocols; 2009; doi:10.1101/pdb.prot5119
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Assay for Human Rad51-Mediated DNA Displacement Loop Formation
Steven Raynard and Patrick Sung
CSH Protocols; 2009; doi:10.1101/pdb.prot5120
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In Vitro Sumoylation of Recombinant Proteins and Subsequent Purification for Use in Enzymatic Assays
Vasupradha Vethantham and James L. Manley
CSH Protocols; 2009; doi:10.1101/pdb.prot5121
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Culturing Lamprey Embryos
Natalya Nikitina, Marianne Bronner-Fraser, and Tatjana Sauka-Spengler
CSH Protocols; 2009; doi:10.1101/pdb.prot5122
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Microinjection of RNA and Morpholino Oligos into Lamprey Embryos
Natalya Nikitina, Marianne Bronner-Fraser, and Tatjana Sauka-Spengler
CSH Protocols; 2009; doi:10.1101/pdb.prot5123
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DiI Cell Labeling in Lamprey Embryos
Natalya Nikitina, Marianne Bronner-Fraser, and Tatjana Sauka-Spengler
CSH Protocols; 2009; doi:10.1101/pdb.prot5124
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Whole-Mount In Situ Hybridization on Lamprey Embryos
Natalya Nikitina, Marianne Bronner-Fraser, and Tatjana Sauka-Spengler
CSH Protocols; 2009; doi:10.1101/pdb.prot5125
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Immunostaining of Whole-Mount and Sectioned Lamprey Embryos
Natalya Nikitina, Marianne Bronner-Fraser, and Tatjana Sauka-Spengler
CSH Protocols; 2009; doi:10.1101/pdb.prot5126
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Fixation and Dissection of Parhyale hawaiensis Embryos
E. Jay Rehm, Roberta L. Hannibal, R. Crystal Chaw, Mario A. Vargas-Vila, and Nipam H. Patel
CSH Protocols; 2009; doi:10.1101/pdb.prot5127
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Injection of Parhyale hawaiensis Blastomeres with Fluorescently Labeled Tracers
E. Jay Rehm, Roberta L. Hannibal, R. Crystal Chaw, Mario A. Vargas-Vila, and Nipam H. Patel
CSH Protocols; 2009; doi:10.1101/pdb.prot5128
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Antibody Staining of Parhyale hawaiensis Embryos
E. Jay Rehm, Roberta L. Hannibal, R. Crystal Chaw, Mario A. Vargas-Vila, and Nipam H. Patel
CSH Protocols; 2009; doi:10.1101/pdb.prot5129
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In Situ Hybridization of Labeled RNA Probes to Fixed Parhyale hawaiensis Embryos
E. Jay Rehm, Roberta L. Hannibal, R. Crystal Chaw, Mario A. Vargas-Vila, and Nipam H. Patel
CSH Protocols; 2009; doi:10.1101/pdb.prot5130
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Preparation of dsRNA for Microinjection Experiments in Mouse
Petr Svoboda
CSH Protocols; 2009; doi:10.1101/pdb.prot5131
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Microinjection of dsRNA into Fully-Grown Mouse Oocytes
Paula Stein
CSH Protocols; 2009; doi:10.1101/pdb.prot5132
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Microinjection of dsRNA into Mouse One-Cell Embryos
Paula Stein
CSH Protocols; 2009; doi:10.1101/pdb.prot5133
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Cloning a Transgene for Transgenic RNAi in Mouse Oocytes
Petr Svoboda
CSH Protocols; 2009; doi:10.1101/pdb.prot5134
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