CSH Protocols, Table of Contents
Volume 3, Issue 2

February 2008

Cover Illustration: Xenopus laevis immunocytochemistry staining showing {alpha}-tubulin (green). Cell nuclei are stained red with propidium iodide. For more information on this method, see Whole-Mount Fluorescence Immunocytochemistry on Xenopus Embryos (doi:10.1101/pdb.prot4957). Image courtesy of Heather Scherr and John Wallingford.

Protocols

PCR Mutagenesis by Overlap Extension and Gene SOE
Abbe N. Vallejo, Robert J. Pogulis, and Larry R. Pease
CSH Protocols; 2008; doi:10.1101/pdb.prot4861
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Preparation of Double-Stranded RNA for Drosophila RNA Interference (RNAi)
Leonie Misquitta, Qin Wei, and Bruce M. Paterson
CSH Protocols; 2008; doi:10.1101/pdb.prot4916
Abstract
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Collection of Drosophila Embryos for RNA Interference (RNAi)
Leonie Misquitta, Qin Wei, and Bruce M. Paterson
CSH Protocols; 2008; doi:10.1101/pdb.prot4917
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Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)
Leonie Misquitta, Qin Wei, and Bruce M. Paterson
CSH Protocols; 2008; doi:10.1101/pdb.prot4918
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Mounting Live Cells Attached to Coverslips for Microscopy
Andrew H. Fischer, Kenneth A. Jacobson, Jack Rose, and Rolf Zeller
CSH Protocols; 2008; doi:10.1101/pdb.prot4927
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Labeling Acetylcholine Receptors in Live Cells Using Rhodamine {alpha}-Bungarotoxin for Imaging
Brad Chazotte
CSH Protocols; 2008; doi:10.1101/pdb.prot4928
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Labeling the Components of the Plasma Membrane with Fluorescent Dyes for Imaging
Brad Chazotte
CSH Protocols; 2008; doi:10.1101/pdb.prot4930
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Labeling the Golgi Apparatus with BODIPY-FL-Ceramide (C5-DMB-ceramide) for Imaging
Brad Chazotte
CSH Protocols; 2008; doi:10.1101/pdb.prot4931
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Calculation of Spot Reliability Evaluation Scores (SRED) for DNA Microarray Data
Kazuro Shimokawa, Rimantas Kodzius, Yonehiro Matsumura, and Yoshihide Hayashizaki
CSH Protocols; 2008; doi:10.1101/pdb.prot4937
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Calculation of Absolute Expression Values for DNA Microarray Data
Kazuro Shimokawa, Rimantas Kodzius, Yonehiro Matsumura, and Yoshihide Hayashizaki
CSH Protocols; 2008; doi:10.1101/pdb.prot4938
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In Vitro Histone Methyltransferase Assay
Ian M. Fingerman, Hai-Ning Du, and Scott D. Briggs
CSH Protocols; 2008; doi:10.1101/pdb.prot4939
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Stable Isotope Labeling of Mammals (SILAM)
Daniel B. McClatchy and John R. Yates III
CSH Protocols; 2008; doi:10.1101/pdb.prot4940
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Fixation, Embedding, and Sectioning of Plant Tissues
Detlef Weigel and Jane Glazebrook
CSH Protocols; 2008; doi:10.1101/pdb.prot4941
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Preparation and Hydrolysis of Digoxygenin-Labeled Probes for In Situ Hybridization of Plant Tissues
Cristina Ferrandiz and Allen Sessions
CSH Protocols; 2008; doi:10.1101/pdb.prot4942
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Nonradioactive In Situ Hybridization of RNA Probes to Sections of Plant Tissues
Cristina Ferrandiz and Allen Sessions
CSH Protocols; 2008; doi:10.1101/pdb.prot4943
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Whole-Mount In Situ Hybridization of RNA Probes to Plant Tissues
Jan Traas
CSH Protocols; 2008; doi:10.1101/pdb.prot4944
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Immunohistochemistry on Sections of Plant Tissues Using Enzyme-Coupled Avidin-Biotin Complex
Detlef Weigel and Jane Glazebrook
CSH Protocols; 2008; doi:10.1101/pdb.prot4945
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Immunohistochemistry on Sections of Plant Tissues Using Alkaline-Phosphatase-Coupled Secondary Antibody
Allen Sessions
CSH Protocols; 2008; doi:10.1101/pdb.prot4946
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Whole-Mount Fluorescence Immunocytochemistry on Xenopus Embryos
Chanjae Lee, Esther Kieserman, Ryan S. Gray, Tae Joo Park, and John Wallingford
CSH Protocols; 2008; doi:10.1101/pdb.prot4957
Abstract
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Information Panels

Drosophila RNA Interference (RNAi) Using a Gal-4 Inducible Transgene Vector
Leonie Misquitta, Qin Wei, and Bruce M. Paterson
CSH Protocols; 2008; doi:10.1101/pdb.ip51
Abstract
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