|
|
|
|
||
Cover Illustration: Fluorescent image of bone-marrow-derived OP9 cells transduced to express HA-tagged Delta-like 1. Cells were grown on poly-L-lysine treated glass coverslips and fixed with paraformaldehyde. The cells were then permeabilized and immunostained with anti-tubulin (green) and anti-HA (red) and overlaid with DAPI nuclear stain (blue). (Image courtesy of Mahmood Mohtashami and Juan Carlos Zúñiga-Pflücker.) For more information on the methods used, see The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro (doi:10.1101/pdb.prot5156).
The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
CSH Protocols; 2009; doi:10.1101/pdb.emo115
Abstract
Full Text
The Choanoflagellates: Heterotrophic Nanoflagellates and Sister Group of the Metazoa
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
CSH Protocols; 2009; doi:10.1101/pdb.emo116
Abstract
Full Text
Basics of Photoactivation
Graham C.R. Ellis-Davies
CSH Protocols; 2009; doi:10.1101/pdb.top55
Abstract
Full Text
In-Gel Trypsin Digest of Gel-Fractionated Proteins
Andrew J. Link and Joshua LaBaer
CSH Protocols; 2009; doi:10.1101/pdb.prot5110
Abstract
Full Text
Culturing the Moss Physcomitrella patens
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
CSH Protocols; 2009; doi:10.1101/pdb.prot5136
Abstract
Full Text
Chromosome Conformation Capture
Nathan F. Cope and Peter Fraser
CSH Protocols; 2009; doi:10.1101/pdb.prot5137
Abstract
Full Text
DNA Extraction from Formalin-Fixed, Paraffin-Embedded Tissue
Weining Tang, Freedom B. David, Malania M. Wilson, Benjamin G. Barwick, Brian R. Leyland-Jones, and Mark M. Bouzyk
CSH Protocols; 2009; doi:10.1101/pdb.prot5138
Abstract
Full Text
Virus-Induced Gene Silencing as a Tool for Delivery of dsRNA into Plants
Meenu Padmanabhan and Savithramma P. Dinesh-Kumar
CSH Protocols; 2009; doi:10.1101/pdb.prot5139
Abstract
Full Text
Isolation and Regeneration of Protoplasts of the Moss Physcomitrella patens
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
CSH Protocols; 2009; doi:10.1101/pdb.prot5140
Abstract
Full Text
Somatic Hybridization in the Moss Physcomitrella patens Using PEG-Induced Protoplast Fusion
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
CSH Protocols; 2009; doi:10.1101/pdb.prot5141
Abstract
Full Text
Chemical and UV Mutagenesis of Spores and Protonemal Tissue from the Moss Physcomitrella patens
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
CSH Protocols; 2009; doi:10.1101/pdb.prot5142
Abstract
Full Text
Transformation of the Moss Physcomitrella patensUsing Direct DNA Uptake by Protoplasts
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
CSH Protocols; 2009; doi:10.1101/pdb.prot5143
Abstract
Full Text
Transformation of the Moss Physcomitrella patens Using T-DNA Mutagenesis
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
CSH Protocols; 2009; doi:10.1101/pdb.prot5144
Abstract
Full Text
Transformation of the Moss Physcomitrella patens Gametophytes Using a Biolistic Projectile Delivery System
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
CSH Protocols; 2009; doi:10.1101/pdb.prot5145
Abstract
Full Text
Isolation of DNA, RNA, and Protein from the Moss Physcomitrella patens Gametophytes
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
CSH Protocols; 2009; doi:10.1101/pdb.prot5146
Abstract
Full Text
Isolation of Single Choanoflagellate Cells from Field Samples and Establishment of Clonal Cultures
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
CSH Protocols; 2009; doi:10.1101/pdb.prot5147
Abstract
Full Text
Starting and Maintaining Monosiga brevicollis Cultures
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
CSH Protocols; 2009; doi:10.1101/pdb.prot5148
Abstract
Full Text
Long-Term Frozen Storage of Choanoflagellate Cultures
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
CSH Protocols; 2009; doi:10.1101/pdb.prot5149
Abstract
Full Text
Visualizing the Subcellular Localization of Actin, ß-Tubulin, and DNA in Monosiga brevicollis
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
CSH Protocols; 2009; doi:10.1101/pdb.prot5150
Abstract
Full Text
Preparation of Total RNA from Monosiga brevicollis and Other Choanoflagellates
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
CSH Protocols; 2009; doi:10.1101/pdb.prot5151
Abstract
Full Text
Rapid Preparation of Genomic DNA from Monosiga brevicollis and Other Choanoflagellates
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
CSH Protocols; 2009; doi:10.1101/pdb.prot5152
Abstract
Full Text
Preparation of High-Molecular-Weight Genomic DNA from Monosiga brevicollis and Other Choanoflagellates
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
CSH Protocols; 2009; doi:10.1101/pdb.prot5153
Abstract
Full Text
Separation of Choanoflagellate and Bacterial Genomic DNA
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
CSH Protocols; 2009; doi:10.1101/pdb.prot5154
Abstract
Full Text
A Simple Free-Radical Polymerization Method for the Fabrication of Microscale Acrylonitrile Fibers for Cellular Probing
Matthew R. Guido
CSH Protocols; 2009; doi:10.1101/pdb.prot5155
Abstract
Full Text
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
Roxanne Holmes and Juan Carlos Zúñiga-Pflücker
CSH Protocols; 2009; doi:10.1101/pdb.prot5156
Abstract
Full Text
     Copyright © 2010 by Cold Spring Harbor Laboratory Press. Online ISSN: 1559-6095 Terms of Service |