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Cover Illustration: Fluorescent image of bone-marrow-derived OP9 cells transduced to express HA-tagged Delta-like 1. Cells were grown on poly-L-lysine treated glass coverslips and fixed with paraformaldehyde. The cells were then permeabilized and immunostained with anti-tubulin (green) and anti-HA (red) and overlaid with DAPI nuclear stain (blue). (Image courtesy of Mahmood Mohtashami and Juan Carlos Zúñiga-Pflücker.) For more information on the methods used, see The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro (doi:10.1101/pdb.prot5156).
The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.emo115
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The Choanoflagellates: Heterotrophic Nanoflagellates and Sister Group of the Metazoa
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Basics of Photoactivation
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In-Gel Trypsin Digest of Gel-Fractionated Proteins
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Culturing the Moss Physcomitrella patens
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Chromosome Conformation Capture
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DNA Extraction from Formalin-Fixed, Paraffin-Embedded Tissue
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Virus-Induced Gene Silencing as a Tool for Delivery of dsRNA into Plants
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Isolation and Regeneration of Protoplasts of the Moss Physcomitrella patens
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5140
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Somatic Hybridization in the Moss Physcomitrella patens Using PEG-Induced Protoplast Fusion
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5141
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Chemical and UV Mutagenesis of Spores and Protonemal Tissue from the Moss Physcomitrella patens
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5142
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Transformation of the Moss Physcomitrella patensUsing Direct DNA Uptake by Protoplasts
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Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5143
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Transformation of the Moss Physcomitrella patens Using T-DNA Mutagenesis
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5144
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Transformation of the Moss Physcomitrella patens Gametophytes Using a Biolistic Projectile Delivery System
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5145
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Isolation of DNA, RNA, and Protein from the Moss Physcomitrella patens Gametophytes
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5146
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Isolation of Single Choanoflagellate Cells from Field Samples and Establishment of Clonal Cultures
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5147
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Starting and Maintaining Monosiga brevicollis Cultures
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5148
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Long-Term Frozen Storage of Choanoflagellate Cultures
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5149
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Visualizing the Subcellular Localization of Actin, ß-Tubulin, and DNA in Monosiga brevicollis
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5150
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Preparation of Total RNA from Monosiga brevicollis and Other Choanoflagellates
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5151
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Rapid Preparation of Genomic DNA from Monosiga brevicollis and Other Choanoflagellates
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5152
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Preparation of High-Molecular-Weight Genomic DNA from Monosiga brevicollis and Other Choanoflagellates
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5153
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Separation of Choanoflagellate and Bacterial Genomic DNA
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5154
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A Simple Free-Radical Polymerization Method for the Fabrication of Microscale Acrylonitrile Fibers for Cellular Probing
Matthew R. Guido
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5155
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The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
Roxanne Holmes and Juan Carlos Zúñiga-Pflücker
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5156
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