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Cover Illustration: Image of adult zebrafish brain sections processed for in situ hybridization against early growth response factor 1 (egr I). Whole-mount brains were hybridized overnight with DIG-labelled antisense RNA, sectioned on a Vibratome, fixed on microscope slides, and treated for 4 h with BCIP/NBT. Images were taken with a Zeiss Axioplan 2 using a 10X objective. Stereoscopic picture of adult zebrafish brain is a composite of approximately 15 photographs assembled with Adobe Photoshop CS3. (Image courtesy of Enrico Kühn and Reinhard Köster.) For more information on this method, see Analysis of Gene Expression by In Situ Hybridization on Adult Zebrafish Brain Sections (doi:10.1101/pdb.prot5382).
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Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.top67
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Live Cell Imaging of Plants
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Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.top68
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Linkage Analysis of Discrete Traits
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Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.top69
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Calculating Linear Flow Velocity from Volumetric Flow Rate
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Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.ip78
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Visualizing Fetal Mouse Vasculature by India Ink Injection
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Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5371
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Calcium Phosphate Transfection of 3T3 Fibroblasts
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Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5372
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DEAE-Dextran Transfection of Lymphocyte Cell Lines
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Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5373
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Transfection by Electroporation of RAW 264.7 Macrophages
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Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5374
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Preparation of the Gel and Column for Chromatofocusing
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Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5375
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Preparation of the Sample for Chromatofocusing: Buffer Exchange and Desalting
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Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5376
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Purification of Proteins by Chromatofocusing
Jamil Mohammad
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5377
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Removal of Elution Buffer from Protein Sample Fractions after Chromatofocusing
Jamil Mohammad
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5378
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Cleaning, Regeneration, and Storage of Chromatofocusing Columns
Jamil Mohammad
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5379
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BiFC Imaging Assay for Plant Protein-Protein Interactions
Yuda Fang and David L. Spector
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5380
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Four-Dimensional Imaging of Plant Cells During the Cell Cycle
Yuda Fang and David L. Spector
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5381
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Analysis of Gene Expression by In Situ Hybridization on Adult Zebrafish Brain Sections
Enrico Kühn and Reinhard Köster
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5382
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Purification of Genomic DNA Extracted from Environmental Sources for Use in a Polymerase Chain Reaction
Molli M. Newman, Jack W. Feminella, and Mark R. Liles
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5383
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DNase-seq: A High-Resolution Technique for Mapping Active Gene Regulatory Elements across the Genome from Mammalian Cells
Lingyun Song and Gregory E. Crawford
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5384
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Genome-Wide Analysis of DNA Synthesis by BrdU Immunoprecipitation on Tiling Microarrays (BrdU-IP-chip) in Saccharomyces cerevisiae
Christopher J. Viggiani, Simon R.V. Knott, and Oscar M. Aparicio
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5385
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Analysis of Nonsense-Mediated mRNA Decay by Monitoring mRNA Half-Lives in Mammalian Cells
Akio Yamashita and Shigeo Ohno
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5386
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