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Cover Illustration: Examples of embryos of Caenorhabditis elegans imaged live by confocal and two-photon microscopy. Center 12 images show stereo pairs of two virtually superimposed embryos (one red, one green) undergoing cleavage divisions. Top four images show three distinct developmentally regulated gene expression patterns, both separately and superimposed in false color. Lower left side image shows muscle- and arcade-cell groups expressing lev-8::gfp and aff-1::gfp, each imaged with a 488-nm laser in distinct embryos. Upper left, upper right, lower right, and bottom six images show live embryos labeled with the vital membrane dye FM 4-64. Upper left and lower right show early cleaving embryos in a maximum intensity projection and a single optical section, respectively. (All embryos measure ~50 × 30 µm.) (Images courtesy of William A. Mohler.) For more information on these methods, see Imaging Embryonic Development in Caenorhabditis elegans (doi:10.1101/pdb.top71).
How Calcium Indicators Work
Stephen R. Adams
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.top70
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Imaging Embryonic Development in Caenorhabditis elegans
William A. Mohler and Ariel B. Isaacson
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.top71
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Baculovirus-Based Display and Gene Delivery Systems
Anna R. Mäkelä, Wolfgang Ernst, Reingard Grabherr, and Christian Oker-Blom
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.top72
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Imaging Single Molecules Using Total Internal Reflection Fluorescence Microscopy (TIRFM)
Samara L. Reck-Peterson, Nathan D. Derr, and Nico Stuurman
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.top73
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Introduction to Linkage Disequilibrium, the HapMap, and Imputation
Benjamin M. Neale
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.top74
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G-less Cassette In Vitro Transcription Using HeLa Cell Nuclear Extracts
Michael F. Carey, Craig L. Peterson, and Stephen T. Smale
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5387
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Suspended Embryo Mount for Imaging Caenorhabditis elegans
William A. Mohler and Ariel B. Isaacson
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5388
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Preparation and Orcein Staining of Mitotic Chromosomes from Drosophila Larval Brain
Sergio Pimpinelli, Silvia Bonaccorsi, Laura Fanti, and Maurizio Gatti
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5389
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Chromosome Banding of Mitotic Chromosomes from Drosophila Larval Brain
Sergio Pimpinelli, Silvia Bonaccorsi, Laura Fanti, and Maurizio Gatti
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5390
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Fluorescent In Situ Hybridization (FISH) of Mitotic Chromosomes from Drosophila Larval Brain
Sergio Pimpinelli, Silvia Bonaccorsi, Laura Fanti, and Maurizio Gatti
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5391
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Using WebGBrowse to Visualize Genome Annotation on GBrowse
Ram Podicheti and Qunfeng Dong
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5392
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Creation of Baculovirus Display Libraries
Anna R. Mäkelä, Wolfgang Ernst, Reingard Grabherr, and Christian Oker-Blom
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5393
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Determination of Recombinant Baculovirus Display Viral Titer
Anna R. Mäkelä, Wolfgang Ernst, Reingard Grabherr, and Christian Oker-Blom
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5394
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Immunofluorescence Analysis of Baculovirus-Displayed Viral Proteins on Infected Insect Cells
Anna R. Mäkelä, Wolfgang Ernst, Reingard Grabherr, and Christian Oker-Blom
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5395
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Immunoelectron Microscopy Analysis of Recombinant Baculovirus Display Viruses
Anna R. Mäkelä, Wolfgang Ernst, Reingard Grabherr, and Christian Oker-Blom
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5396
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Monitoring Baculovirus-Mediated Efficiency of Gene Delivery
Anna R. Mäkelä, Wolfgang Ernst, Reingard Grabherr, and Christian Oker-Blom
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5397
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Determining Single-Molecule Intensity as a Function of Power Density
Samara L. Reck-Peterson, Nathan D. Derr, and Nico Stuurman
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5398
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Imaging Single Molecular Motor Motility with Total Internal Reflection Fluorescence Microscopy (TIRFM)
Samara L. Reck-Peterson, Nathan D. Derr, and Nico Stuurman
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5399
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Unbiased Identification of Protein-Bait Interactions Using Biochemical Enrichment and Quantitative Proteomics
Shao-En Ong
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5400
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Overexpressing Tagged Proteins in Plants Using a Modified Gateway Cloning Strategy
Manu J. Dubin, Chris Bowler, and Giovanna Benvenuto
Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.prot5401
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