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Cover Illustration: Multicolor labeling of embryonic day 14 mouse cortex using seven combinations of the carbocyanine dyes DiI, DiO, and DiD delivered ballistically. The brain was fixed with paraformaldehyde, sectioned by a vibratome, and then ballistically labeled using a gene gun. (Image reproduced from Gan, W.B., Grutzendler, J., Wong, W.T., Wong, R.O.L., and Lichtman, J.W. 2000. Multicolor “DiOlistic” labeling of neuronal circuits using lipophilic dye combinations. Neuron 27: 219-225. Permission to reproduce granted by Elsevier © 2000.) For more information on the methods used, see Ballistic Delivery of Dyes for Structural and Functional Studies of the Nervous System (doi:10.1101/pdb.prot5202).
The Snail Ilyanassa: A Reemerging Model for Studies in Development
Maey Gharbiah, James Cooley, Esther M. Leise, Ayaki Nakamoto, Jeremy S. Rabinowitz, J. David Lambert, and Lisa M. Nagy
CSH Protocols; 2009; doi:10.1101/pdb.emo120
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Helobdella (Leech): A Model for Developmental Studies
David A. Weisblat and Dian-Han Kuo
CSH Protocols; 2009; doi:10.1101/pdb.emo121
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Optimization and Troubleshooting in PCR
Kenneth H. Roux
CSH Protocols; 2009; doi:10.1101/pdb.ip66
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Reconstitution of Nucleosomal Arrays Using Recombinant Drosophila ACF and NAP1
Craig L. Peterson
CSH Protocols; 2009; doi:10.1101/pdb.prot5114
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Purification of Recombinant Drosophila ACF
Craig L. Peterson
CSH Protocols; 2009; doi:10.1101/pdb.prot5115
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Purification of Recombinant Drosophila NAP1
Craig L. Peterson
CSH Protocols; 2009; doi:10.1101/pdb.prot5116
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Obtaining Ilyanassa Snail Embryos
Maey Gharbiah, James Cooley, Esther M. Leise, Ayaki Nakamoto, Jeremy S. Rabinowitz, J. David Lambert, and Lisa M. Nagy
CSH Protocols; 2009; doi:10.1101/pdb.prot5183
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Induction of Larval Metamorphosis in the Snail Ilyanassa
Maey Gharbiah, James Cooley, Esther M. Leise, Ayaki Nakamoto, Jeremy S. Rabinowitz, J. David Lambert, and Lisa M. Nagy
CSH Protocols; 2009; doi:10.1101/pdb.prot5184
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Pressure Injection of Ilyanassa Snail Embryos
Maey Gharbiah, James Cooley, Esther M. Leise, Ayaki Nakamoto, Jeremy S. Rabinowitz, J. David Lambert, and Lisa M. Nagy
CSH Protocols; 2009; doi:10.1101/pdb.prot5185
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Fixation of Ilyanassa Snail Embryos and Larvae
Maey Gharbiah, James Cooley, Esther M. Leise, Ayaki Nakamoto, Jeremy S. Rabinowitz, J. David Lambert, and Lisa M. Nagy
CSH Protocols; 2009; doi:10.1101/pdb.prot5186
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Isolation of Genomic DNA from Ilyanassa Snail Larvae
Maey Gharbiah, James Cooley, Esther M. Leise, Ayaki Nakamoto, Jeremy S. Rabinowitz, J. David Lambert, and Lisa M. Nagy
CSH Protocols; 2009; doi:10.1101/pdb.prot5187
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Isolating Protein from Ilyanassa Snail Embryos
Maey Gharbiah, James Cooley, Esther M. Leise, Ayaki Nakamoto, Jeremy S. Rabinowitz, J. David Lambert, and Lisa M. Nagy
CSH Protocols; 2009; doi:10.1101/pdb.prot5188
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Handling of Helobdella (Leech) Embryos
David A. Weisblat and Dian-Han Kuo
CSH Protocols; 2009; doi:10.1101/pdb.prot5189
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Microinjection of Helobdella (Leech) Embryos
David A. Weisblat and Dian-Han Kuo
CSH Protocols; 2009; doi:10.1101/pdb.prot5190
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Devitellinization of Living Helobdella (Leech) Embryos
David A. Weisblat and Dian-Han Kuo
CSH Protocols; 2009; doi:10.1101/pdb.prot5191
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Arnolds’ Silver Staining of Helobdella (Leech) Embryos
David A. Weisblat and Dian-Han Kuo
CSH Protocols; 2009; doi:10.1101/pdb.prot5192
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Immunostaining Helobdella (Leech) Embryos
David A. Weisblat and Dian-Han Kuo
CSH Protocols; 2009; doi:10.1101/pdb.prot5193
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In Situ Hybridization of Helobdella (Leech) Embryos
David A. Weisblat and Dian-Han Kuo
CSH Protocols; 2009; doi:10.1101/pdb.prot5194
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Whole-Mount Preparation of Helobdella (Leech) Embryos for Microscopy
David A. Weisblat and Dian-Han Kuo
CSH Protocols; 2009; doi:10.1101/pdb.prot5195
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tDNA-PCR Followed by Automated Fluorescent Capillary Electrophoresis for Identification of Bacterial Species
Margo Baele, Tim Stakenborg, Guido Lopes dos Santos Santiago, Freddy Haesebrouck, and Mario Vaneechoutte
CSH Protocols; 2009; doi:10.1101/pdb.prot5196
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Enzymatic Synthesis of Multi-Milligram Quantities of Large, Linear DNA Molecules for Structural Studies
David Jeruzalmi
CSH Protocols; 2009; doi:10.1101/pdb.prot5197
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Quick Preparation of Genomic DNA from Drosophila
Audrey M. Huang, E. Jay Rehm, and Gerald M. Rubin
CSH Protocols; 2009; doi:10.1101/pdb.prot5198
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Recovery of DNA Sequences Flanking P-Element Insertions in Drosophila: Inverse PCR and Plasmid Rescue
Audrey M. Huang, E. Jay Rehm, and Gerald M. Rubin
CSH Protocols; 2009; doi:10.1101/pdb.prot5199
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Dye Loading with Patch Pipettes
Jens Eilers and Arthur Konnerth
CSH Protocols; 2009; doi:10.1101/pdb.prot5201
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Ballistic Delivery of Dyes for Structural and Functional Studies of the Nervous System
Wen-Biao Gan, Jaime Grutzendler, Rachel O. Wong, and Jeff W. Lichtman
CSH Protocols; 2009; doi:10.1101/pdb.prot5202
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