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Cover Illustration: Fluorescence montage of Caenorhabditis elegans from a one-cell zygote to a threefold embryo. Embryos were placed on a poly-L-lysine treated slide, freeze-cracked to remove the shell and fixed with methanol/acetone. The embryos were then immunostained with anti-PGL-1 to show P granules (green), mAb414 to show nuclear pores (red), and DAPI nuclear stain (blue). (Image courtesy of Dustin Updike and Susan Strome.) For more information on antibody staining in C. elegans, see Preparation and Immunolabeling of Caenorhabditis elegans (doi:10.1101/pdb.prot5216).
The Genus Polypterus (Bichirs): A Fish Group Diverged at the Stem of Ray-Finned Fishes (Actinopterygii)
Masaki Takeuchi, Masataka Okabe, and Shinichi Aizawa
CSH Protocols; 2009; doi:10.1101/pdb.emo117
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The African Butterfly Bicyclus anynana: A Model for Evolutionary Genetics and Evolutionary Developmental Biology
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
CSH Protocols; 2009; doi:10.1101/pdb.emo122
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Construction and Characterization of Adenovirus Vectors
P. Joel Ross and Robin J. Parks
CSH Protocols; 2009; doi:10.1101/pdb.prot5011
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Microinjection of Bichir (Polypterus) Embryos
Masaki Takeuchi, Masataka Okabe, and Shinichi Aizawa
CSH Protocols; 2009; doi:10.1101/pdb.prot5157
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Whole-Mount In Situ Hybridization of Bichir (Polypterus) Embryos
Masaki Takeuchi, Masataka Okabe, and Shinichi Aizawa
CSH Protocols; 2009; doi:10.1101/pdb.prot5158
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Computational Docking of Biomolecular Complexes with AutoDock
David S. Goodsell
CSH Protocols; 2009; doi:10.1101/pdb.prot5200
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Culture and Propagation of Laboratory Populations of the African Butterfly Bicyclus anynana
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
CSH Protocols; 2009; doi:10.1101/pdb.prot5203
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Surgical Manipulations on Pupal Wings from the African Butterfly Bicyclus anynana: Damage and Cauteries
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
CSH Protocols; 2009; doi:10.1101/pdb.prot5204
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Surgical Manipulations on Pupal Wings from the African Butterfly Bicyclus anynana: Grafts
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
CSH Protocols; 2009; doi:10.1101/pdb.prot5205
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Fixation and Dissection of Embryos from the African Butterfly Bicyclus anynana
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
CSH Protocols; 2009; doi:10.1101/pdb.prot5206
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Dissection of Larval and Pupal Wings from the African Butterfly Bicyclus anynana
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
CSH Protocols; 2009; doi:10.1101/pdb.prot5207
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In Situ Hybridization of Embryos and Larval and Pupal Wings from the African Butterfly Bicyclus anynana
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
CSH Protocols; 2009; doi:10.1101/pdb.prot5208
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Immunohistochemistry Staining of Embryos from the African Butterfly Bicyclus anynana
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
CSH Protocols; 2009; doi:10.1101/pdb.prot5209
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Immunohistochemistry Staining of Wing Discs from the African Butterfly Bicyclus anynana
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
CSH Protocols; 2009; doi:10.1101/pdb.prot5210
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Extraction and Gas Chromatography Analysis of Adult Pheromones from the African Butterfly Bicyclus anynana
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
CSH Protocols; 2009; doi:10.1101/pdb.prot5211
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Fresh Weight, Dry Weight, and Fat Content of Adult African Butterflies Bicyclus anynana
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
CSH Protocols; 2009; doi:10.1101/pdb.prot5212
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Constant Volume Respirometry in the African Butterfly Bicyclus anynana
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
CSH Protocols; 2009; doi:10.1101/pdb.prot5213
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Hemolymph Extraction from Various Developmental Stages of the African Butterfly Bicyclus anynana
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
CSH Protocols; 2009; doi:10.1101/pdb.prot5214
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Injection of Chemicals into Pupae of the African Butterfly Bicyclus anynana
Paul M. Brakefield, Patrícia Beldade, and Bas J. Zwaan
CSH Protocols; 2009; doi:10.1101/pdb.prot5215
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Preparation and Immunolabeling of Caenorhabditis elegans
Sarah Crittenden and Judith Kimble
CSH Protocols; 2009; doi:10.1101/pdb.prot5216
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Construction of Adenovirus Vectors with RGD-Modified Fiber for Transductional Targeting
Yosuke Kawakami and David T. Curiel
CSH Protocols; 2009; doi:10.1101/pdb.prot5217
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Construction of Fusion Proteins for Transductional Targeting
Yosuke Kawakami and David T. Curiel
CSH Protocols; 2009; doi:10.1101/pdb.prot5218
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Construction of Adenovirus Vectors for Transcriptional Targeting
Yosuke Kawakami and David T. Curiel
CSH Protocols; 2009; doi:10.1101/pdb.prot5219
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Comet Fluorescence In Situ Hybridization (Comet-FISH): Detection of DNA Damage
Wiebke Schlörmann and Michael Glei
CSH Protocols; 2009; doi:10.1101/pdb.prot5220
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Systematic Monitoring of Protein Complex Composition and Abundance by Blue-Native PAGE
Holger Eubel and A. Harvey Millar
CSH Protocols; 2009; doi:10.1101/pdb.prot5221
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Metabolic Footprinting for the Study of Microbial Biodiversity
Georgina A. Pope, Donald A. MacKenzie, Marianne Defernez, and Ian N. Roberts
CSH Protocols; 2009; doi:10.1101/pdb.prot5222
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