Cold Spring Harbor Protocols, Table of Contents
Volume 3, Issue 7

July 2008

Cover Illustration: Section of renal medulla from an adult murine kidney showing gene and protein expression in the nephron tubules. Uromodulin gene expression in distal straight tubules (blue) is detected by RNA in situ hybridization, with dual immunohistochemistry to detect Aquaporin 1 protein expression in thin descending limb tubules of the Loop of Henle (orange). (Image courtesy of Bree Rumballe, Kylie Georgas, and Melissa H. Little.) For more information on this method, see High-Throughput Paraffin Section In Situ Hybridization and Dual Immunohistochemistry on Mouse Tissues (doi:10.1101/pdb.prot5030).

Protocols

Isolation and Culture of Mouse Keratinocytes
Jonathan C.R. Jones
CSH Protocols; 2008; doi:10.1101/pdb.prot4476
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Identification of Differential Genes by Suppression Subtractive Hybridization: I. Preparation of Subtracted cDNA or Genomic DNA Library
Denis V. Rebrikov
CSH Protocols; 2008; doi:10.1101/pdb.prot4855
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Identification of Differential Genes by Suppression Subtractive Hybridization: II. Subtractive Hybridization
Denis V. Rebrikov
CSH Protocols; 2008; doi:10.1101/pdb.prot4856
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Identification of Differential Genes by Suppression Subtractive Hybridization: III. PCR Amplification of Differentially Presented DNAs
Denis V. Rebrikov
CSH Protocols; 2008; doi:10.1101/pdb.prot4857
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Identification of Differential Genes by Suppression Subtractive Hybridization: IV. Mirror Orientation Selection (MOS)
Denis V. Rebrikov
CSH Protocols; 2008; doi:10.1101/pdb.prot4858
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Identification of Differential Genes by Suppression Subtractive Hybridization: V. PCR-Based DNA Dot Blot
Denis V. Rebrikov
CSH Protocols; 2008; doi:10.1101/pdb.prot4859
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Identification of Differential Genes by Suppression Subtractive Hybridization: VI. Differential Hybridization with Tester and Driver DNA Probes
Denis V. Rebrikov
CSH Protocols; 2008; doi:10.1101/pdb.prot4860
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Phenotypic Analysis of Arabidopsis Mutants: Flowering Time
Miguel Blázquez
CSH Protocols; 2008; doi:10.1101/pdb.prot4963
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Browsing HapMap Data Using the Genome Browser
Albert Vernon Smith
CSH Protocols; 2008; doi:10.1101/pdb.prot5023
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Generating HapMap Data Text Reports Using the Genome Browser
Albert Vernon Smith
CSH Protocols; 2008; doi:10.1101/pdb.prot5024
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Manipulating HapMap Data Using HaploView
Albert Vernon Smith
CSH Protocols; 2008; doi:10.1101/pdb.prot5025
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Retrieving HapMap Data Using HapMart
Albert Vernon Smith
CSH Protocols; 2008; doi:10.1101/pdb.prot5026
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Retrieving HapMap Data via Bulk Download
Albert Vernon Smith
CSH Protocols; 2008; doi:10.1101/pdb.prot5027
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In Vitro Screening for Regulated Transcription Factors with Differential Display of DNA-Binding Proteins (DDDP)
Hans Reinke and Ueli Schibler
CSH Protocols; 2008; doi:10.1101/pdb.prot5028
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Generation of Driver and Reporter Constructs for the GAL4 Expression System in Drosophila
Tony D. Southall and Andrea H. Brand
CSH Protocols; 2008; doi:10.1101/pdb.prot5029
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High-Throughput Paraffin Section In Situ Hybridization and Dual Immunohistochemistry on Mouse Tissues
Bree Rumballe, Kylie Georgas, and Melissa H. Little
CSH Protocols; 2008; doi:10.1101/pdb.prot5030
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Topic Introductions

Identification of Differential Genes by Suppression Subtractive Hybridization: An Overview
Denis V. Rebrikov
CSH Protocols; 2008; doi:10.1101/pdb.top21
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The GAL4 System: A Versatile Toolkit for Gene Expression in Drosophila
Tony D. Southall, David A. Elliott, and Andrea H. Brand
CSH Protocols; 2008; doi:10.1101/pdb.top49
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Nuclear Stains
John Alan Kiernan
CSH Protocols; 2008; doi:10.1101/pdb.top50
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Anionic Counterstains
John Alan Kiernan
CSH Protocols; 2008; doi:10.1101/pdb.top51
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