Cold Spring Harbor Protocols, Table of Contents
Volume 2008, Issue 7

July 2008

Cover Illustration: Section of renal medulla from an adult murine kidney showing gene and protein expression in the nephron tubules. Uromodulin gene expression in distal straight tubules (blue) is detected by RNA in situ hybridization, with dual immunohistochemistry to detect Aquaporin 1 protein expression in thin descending limb tubules of the Loop of Henle (orange). (Image courtesy of Bree Rumballe, Kylie Georgas, and Melissa H. Little.) For more information on this method, see High-Throughput Paraffin Section In Situ Hybridization and Dual Immunohistochemistry on Mouse Tissues (doi:10.1101/pdb.prot5030).

Protocols

Isolation and Culture of Mouse Keratinocytes
Jonathan C.R. Jones
CSH Protocols; 2008; doi:10.1101/pdb.prot4476
Abstract
Full Text

Identification of Differential Genes by Suppression Subtractive Hybridization: I. Preparation of Subtracted cDNA or Genomic DNA Library
Denis V. Rebrikov
CSH Protocols; 2008; doi:10.1101/pdb.prot4855
Abstract
Full Text

Identification of Differential Genes by Suppression Subtractive Hybridization: II. Subtractive Hybridization
Denis V. Rebrikov
CSH Protocols; 2008; doi:10.1101/pdb.prot4856
Abstract
Full Text

Identification of Differential Genes by Suppression Subtractive Hybridization: III. PCR Amplification of Differentially Presented DNAs
Denis V. Rebrikov
CSH Protocols; 2008; doi:10.1101/pdb.prot4857
Abstract
Full Text

Identification of Differential Genes by Suppression Subtractive Hybridization: IV. Mirror Orientation Selection (MOS)
Denis V. Rebrikov
CSH Protocols; 2008; doi:10.1101/pdb.prot4858
Abstract
Full Text

Identification of Differential Genes by Suppression Subtractive Hybridization: V. PCR-Based DNA Dot Blot
Denis V. Rebrikov
CSH Protocols; 2008; doi:10.1101/pdb.prot4859
Abstract
Full Text

Identification of Differential Genes by Suppression Subtractive Hybridization: VI. Differential Hybridization with Tester and Driver DNA Probes
Denis V. Rebrikov
CSH Protocols; 2008; doi:10.1101/pdb.prot4860
Abstract
Full Text

Phenotypic Analysis of Arabidopsis Mutants: Flowering Time
Miguel Blázquez
CSH Protocols; 2008; doi:10.1101/pdb.prot4963
Abstract
Full Text

Browsing HapMap Data Using the Genome Browser
Albert Vernon Smith
CSH Protocols; 2008; doi:10.1101/pdb.prot5023
Abstract
Full Text

Generating HapMap Data Text Reports Using the Genome Browser
Albert Vernon Smith
CSH Protocols; 2008; doi:10.1101/pdb.prot5024
Abstract
Full Text

Manipulating HapMap Data Using HaploView
Albert Vernon Smith
CSH Protocols; 2008; doi:10.1101/pdb.prot5025
Abstract
Full Text

Retrieving HapMap Data Using HapMart
Albert Vernon Smith
CSH Protocols; 2008; doi:10.1101/pdb.prot5026
Abstract
Full Text

Retrieving HapMap Data via Bulk Download
Albert Vernon Smith
CSH Protocols; 2008; doi:10.1101/pdb.prot5027
Abstract
Full Text

In Vitro Screening for Regulated Transcription Factors with Differential Display of DNA-Binding Proteins (DDDP)
Hans Reinke and Ueli Schibler
CSH Protocols; 2008; doi:10.1101/pdb.prot5028
Abstract
Full Text

Generation of Driver and Reporter Constructs for the GAL4 Expression System in Drosophila
Tony D. Southall and Andrea H. Brand
CSH Protocols; 2008; doi:10.1101/pdb.prot5029
Abstract
Full Text

High-Throughput Paraffin Section In Situ Hybridization and Dual Immunohistochemistry on Mouse Tissues
Bree Rumballe, Kylie Georgas, and Melissa H. Little
CSH Protocols; 2008; doi:10.1101/pdb.prot5030
Abstract
Full Text

Topic Introductions

Identification of Differential Genes by Suppression Subtractive Hybridization: An Overview
Denis V. Rebrikov
CSH Protocols; 2008; doi:10.1101/pdb.top21
Abstract
Full Text

The GAL4 System: A Versatile Toolkit for Gene Expression in Drosophila
Tony D. Southall, David A. Elliott, and Andrea H. Brand
CSH Protocols; 2008; doi:10.1101/pdb.top49
Abstract
Full Text

Nuclear Stains
John Alan Kiernan
CSH Protocols; 2008; doi:10.1101/pdb.top50
Abstract
Full Text

Anionic Counterstains
John Alan Kiernan
CSH Protocols; 2008; doi:10.1101/pdb.top51
Abstract
Full Text