CSH Protocols, Table of Contents
Volume 3, Issue 8

August 2008

Cover Illustration: Whole mount in situ hybridization of a 36-h post-fertilization zebrafish embryo with a probe for Zcoe2, the zebrafish homologue of EBF-2, a transcription factor involved in neuronal differentiation. Dorsal view, mounted in 90% glycerol. NBT/BCIP was used as the substrate for the chromogenic reaction. (Image courtesy of Katrin Volkmann and Reinhard Köster, Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Developmental Genetics, Neuherberg). For more information on this method, see Whole Mount RNA In Situ Hybridization of Zebrafish Embryos: Probe Synthesis (doi:10.1101/pdb.prot5036), Whole Mount RNA In Situ Hybridization of Zebrafish Embryos: Hybridization (doi:10.1101/pdb.prot5037), and Whole Mount RNA In Situ Hybridization of Zebrafish Embryos: Mounting (doi:10.1101/pdb.prot5038).

Protocols

Inoculation and Passaging of Mammalian Monolayer Cell Cultures
Gretchen J. Darlington
CSH Protocols; 2008; doi:10.1101/pdb.prot4347
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Quantifying Protein by Bicinchoninic Acid
Richard J. Simpson
CSH Protocols; 2008; doi:10.1101/pdb.prot4722
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The PAS Reaction for Staining Cell Walls
Stuart Baum
CSH Protocols; 2008; doi:10.1101/pdb.prot4956
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Phenotypic Analysis of Arabidopsis Mutants: Trypan Blue Stain for Fungi, Oomycetes, and Dead Plant Cells
Saskia van Wees
CSH Protocols; 2008; doi:10.1101/pdb.prot4982
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Cryosectioning Tissues
Andrew H. Fischer, Kenneth A. Jacobson, Jack Rose, and Rolf Zeller
CSH Protocols; 2008; doi:10.1101/pdb.prot4991
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Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version A
Gustavo Tiscornia, Oded Singer, and Inder M. Verma
CSH Protocols; 2008; doi:10.1101/pdb.prot5009
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Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version B
Gustavo Tiscornia, Oded Singer, and Inder M. Verma
CSH Protocols; 2008; doi:10.1101/pdb.prot5010
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Phage-Display Methodology for the Study of Protein-Protein Interactions: Stage 1, Generating Libraries
Carl S. Goodyear and Gregg J. Silverman
CSH Protocols; 2008; doi:10.1101/pdb.prot5020
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Phage-Display Methodology for the Study of Protein-Protein Interactions: Stage 2, Panning
Carl S. Goodyear and Gregg J. Silverman
CSH Protocols; 2008; doi:10.1101/pdb.prot5021
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Phage-Display Methodology for the Study of Protein-Protein Interactions: Stage 3, Binding Analysis
Carl S. Goodyear and Gregg J. Silverman
CSH Protocols; 2008; doi:10.1101/pdb.prot5022
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Analyzing DNA Replication I: Labeling Animals, Tissues, and Cells with Bromodeoxyuridine (BrdU)
Dean Jackson and Peter R. Cook
CSH Protocols; 2008; doi:10.1101/pdb.prot5031
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Analyzing DNA Replication II: Fixation and Processing of Tissues and Cells Labeled with Bromodeoxyuridine (BrdU)
Dean Jackson and Peter R. Cook
CSH Protocols; 2008; doi:10.1101/pdb.prot5032
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Analyzing DNA Replication III: Antibody Labeling of Incorporated Bromodeoxyuridine (BrdU) in Tissues and Cell
Dean Jackson and Peter R. Cook
CSH Protocols; 2008; doi:10.1101/pdb.prot5033
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In Vitro Assays for the Extracellular Matrix Protein-Regulated Extravasation Process
Chaoyu Ma and Xiao-Fan Wang
CSH Protocols; 2008; doi:10.1101/pdb.prot5034
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Whole Mount RNA In Situ Hybridization on Zebrafish Embryos: Probe Synthesis
Cecilia Moens
CSH Protocols; 2008; doi:10.1101/pdb.prot5036
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Whole Mount RNA In Situ Hybridization on Zebrafish Embryos: Hybridization
Cecilia Moens
CSH Protocols; 2008; doi:10.1101/pdb.prot5037
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Whole Mount RNA In Situ Hybridization on Zebrafish Embryos: Mounting
Cecilia Moens
CSH Protocols; 2008; doi:10.1101/pdb.prot5038
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Isolation of Centrosomes from Cultured Mammalian Cells
Thomas E. Meigs and Daniel D. Kaplan
CSH Protocols; 2008; doi:10.1101/pdb.prot5039
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Topic Introductions

Imaging Calcium Dynamics Using Targeted Recombinant Aequorins
Tullio Pazzan and Rosario Rizzuto
CSH Protocols; 2008; doi:10.1101/pdb.top26
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Phage-Display Methodology for the Study of Protein-Protein Interactions: Overview
Carl S. Goodyear and Gregg J. Silverman
CSH Protocols; 2008; doi:10.1101/pdb.top48
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