Cold Spring Harbor Protocols, Table of Contents
Volume 2009, Issue 8

August 2009

Cover Illustration: Fluorescence image of a mouse embryonic stem cell line expressing human EphA4 that was established using the Sleeping Beauty transposon system. Cells were grown in neuronal differentiation media for 4 d, fixed and labeled with a TUJ1 (neurons; green) antibody and stained with Hoescht dye (nuclei; blue). Image was taken with a Leica inverted microscope (DMI 3000B) using a 20X objective. (Image courtesy of Ann Davidson and Catherine Krull.) For more information on this method, see Use of the Sleeping Beauty Transposon System for Stable Gene Expression in Mouse Embryonic Stem Cells (doi:10.1101/pdb.prot5270).

Emerging Model Organisms

The Red Flour Beetle, Tribolium castaneum (Coleoptera): A Model for Studies of Development and Pest Biology
Susan J. Brown, Teresa D. Shippy, Sherry Miller, Renata Bolognesi, Richard W. Beeman, Marcé D. Lorenzen, Gregor Bucher, Ernst A. Wimmer, and Martin Klingler
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.emo126
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Morphology and Husbandry of the Large Milkweed Bug, Oncopeltus fasciatus
Paul Liu and Thomas C. Kaufman
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.emo127
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Ambystoma mexicanum, the Axolotl: A Versatile Amphibian Model for Regeneration, Development, and Evolution Studies
S. Randal Voss, Hans H. Epperlein, and Elly M. Tanaka
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.emo128
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Topic Introductions

Assessing Significance in Genetic Association Studies
Mark J. Daly
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.top58
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Engineering the Surface Glycoproteins of Lentiviral Vectors for Targeted Gene Transfer
Els Verhoeyen and François-Loïc Cosset
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.top59
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Information Panel

Strategies for Cloning PCR Products
Peter D’Arpa
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.ip68
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Protocols

RNAi in the Red Flour Beetle (Tribolium)
Nico Posnien, Johannes Schinko, Daniela Grossmann, Teresa D. Shippy, Barbora Konopova, and Gregor Bucher
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5256
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Concurrent In Situ Hybridization and Antibody Staining in Red Flour Beetle (Tribolium) Embryos
Teresa D. Shippy, Cassondra M. Coleman, Yoshinori Tomoyasu, and Susan J. Brown
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5257
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Single and Double Whole-Mount In Situ Hybridization in Red Flour Beetle (Tribolium) Embryos
Johannes Schinko, Nico Posnien, Sebastian Kittelmann, Nikolaus Koniszewski, and Gregor Bucher
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5258
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Red Flour Beetle (Tribolium) Germline Transformation and Insertional Mutagenesis
Andreas J. Berghammer, Markus Weber, Jochen Trauner, and Martin Klingler
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5259
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Analyzing Microarray Data Using WebArray
Yipeng Wang, Michael McClelland, and Xiao-Qin Xia
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5260
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Dissection and Fixation of Large Milkweed Bug (Oncopeltus) Embryos
Paul Liu and Thomas C. Kaufman
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5261
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In Situ Hybridization of Large Milkweed Bug (Oncopeltus) Tissues
Paul Liu and Thomas C. Kaufman
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5262
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Axolotl (Ambystoma mexicanum) In Vitro Fertilization
Shahryar Khattak and Elly M. Tanaka
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5263
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Generation of Transgenic Axolotls (Ambystoma mexicanum)
Shahryar Khattak, Tobias Richter, and Elly M. Tanaka
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5264
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Axolotl (Ambystoma mexicanum) Embryonic Transplantation Methods
Eugen Nacu, Dunja Knapp, Elly M. Tanaka, and Hans H. Epperlein
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5265
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Grafting Axolotl (Ambystoma mexicanum) Limb Skin and Cartilage from GFP+ Donors to Normal Hosts
Martin Kragl and Elly M. Tanaka
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5266
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Axolotl (Ambystoma mexicanum) Limb and Tail Amputation
Martin Kragl and Elly M. Tanaka
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5267
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Induction of Metamorphosis in Axolotls (Ambystoma mexicanum)
Robert B. Page and S. Randal Voss
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5268
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Chromosome Orientation Fluorescence In Situ Hybridization (CO-FISH)
Eli S. Williams and Susan M. Bailey
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5269
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Use of the Sleeping Beauty Transposon System for Stable Gene Expression in Mouse Embryonic Stem Cells
Ann E. Davidson, Theresa E. Gratsch, Maria H. Morell, K. Sue O’Shea, and Catherine E. Krull
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5270
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Isolation and Cloning of High-Molecular-Weight Metagenomic DNA from Soil Microorganisms
Mark R. Liles, Lynn L. Williamson, Jitsupang Rodbumrer, Vigdis Torsvik, Larissa C. Parsley, Robert M. Goodman, and Jo Handelsman
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5271
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Preparation of Coverslips for Neuronal Cultures
Cristóvão Albuquerque, Donald J. Joseph, Papiya Choudhury, and Amy B. MacDermott
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5272
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Dissection, Plating, and Maintenance of Cortical Astrocyte Cultures
Cristóvão Albuquerque, Donald J. Joseph, Papiya Choudhury, and Amy B. MacDermott
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5273
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Dissection, Plating, and Maintenance of Dorsal Horn Neuron Cultures
Cristóvão Albuquerque, Donald J. Joseph, Papiya Choudhury, and Amy B. MacDermott
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5274
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Dissection, Plating, and Maintenance of Dorsal Root Ganglion Neurons for Monoculture and for Coculture with Dorsal Horn Neurons
Cristóvão Albuquerque, Donald J. Joseph, Papiya Choudhury, and Amy B. MacDermott
Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5275
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