CSH Protocols, Table of Contents
Volume 3, Issue 9

September 2008

Cover Illustration: Phase contrast image of undifferentiated human embryonic stem cell colony (HSF6 line). Cells were cultured on irradiated mouse embryonic fibroblast feeder cells. (Image courtesy of Sohyun L. McElroy and Renee A. Reijo Pera.) For more information on this method, see Culturing Human Embryonic Stem Cells with Mouse Embryonic Fibroblast Feeder Cells (doi:10.1101/pdb.prot5042).

Protocols

Estimation of Free Thiols and Disulfide Bonds Using Ellman's Reagent
Richard J. Simpson
CSH Protocols; 2008; doi:10.1101/pdb.prot4699
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Subbing Slides
Carol Ann Paul, Barbara Beltz, and Joanne Berger-Sweeney
CSH Protocols; 2008; doi:10.1101/pdb.prot4804
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The Nissl Stain: A Stain for Cell Bodies in Brain Sections
Carol Ann Paul, Barbara Beltz, and Joanne Berger-Sweeney
CSH Protocols; 2008; doi:10.1101/pdb.prot4805
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Analyzing Glucose Phosphate Isomerase Isozymes in Chimeric Mouse Tissues by Electrophoresis
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2008; doi:10.1101/pdb.prot4813
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Clearing Plant Tissue for Observation of Vascular Strands
Sioux Christensen
CSH Protocols; 2008; doi:10.1101/pdb.prot4952
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Phenotypic Analysis of Arabidopsis Mutants: Heavy Metal Stress
David Lee and Julian Schroeder
CSH Protocols; 2008; doi:10.1101/pdb.prot4971
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Streamlined Protocol for Antibody Staining of Large Numbers of Samples in Drosophila
Ilaria Rebay and Richard G. Fehon
CSH Protocols; 2008; doi:10.1101/pdb.prot5000
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Human Lymphocyte Culture and Chromosome Analysis
Peter Benn and Judith Delach
CSH Protocols; 2008; doi:10.1101/pdb.prot5035
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Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
Sohyun L. McElroy and Renee A. Reijo Pera
CSH Protocols; 2008; doi:10.1101/pdb.prot5040
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Preparation of Mouse Embryonic Fibroblast Feeder Cells for Human Embryonic Stem Cell Culture
Sohyun L. McElroy and Renee A. Reijo Pera
CSH Protocols; 2008; doi:10.1101/pdb.prot5041
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Culturing Human Embryonic Stem Cells with Mouse Embryonic Fibroblast Feeder Cells
Sohyun L. McElroy and Renee A. Reijo Pera
CSH Protocols; 2008; doi:10.1101/pdb.prot5042
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Preparation of Human Foreskin Fibroblasts for Human Embryonic Stem Cell Culture
Sarita Panula and Renee A.Reijo Pera
CSH Protocols; 2008; doi:10.1101/pdb.prot5043
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Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
Sohyun L. McElroy and Renee A. Reijo Pera
CSH Protocols; 2008; doi:10.1101/pdb.prot5044
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Method for Single-Cell Sorting and Expansion of Genetically Modified Human Embryonic Stem Cells
Cory R. Nicholas and Renee A. Reijo Pera
CSH Protocols; 2008; doi:10.1101/pdb.prot5045
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DNA Methylation Analysis of Human Imprinted Loci by Bisulfite Genomic Sequencing
Vanessa T. Angeles and Renee A. Reijo Pera
CSH Protocols; 2008; doi:10.1101/pdb.prot5046
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Metaphase Spreads and Spectral Karyotyping of Human Embryonic Stem Cells
Ha Nam Nguyen and Renee A. Reijo Pera
CSH Protocols; 2008; doi:10.1101/pdb.prot5047
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Human Germ Cell Lineage Differentiation from Embryonic Stem Cells
Kehkooi Kee and Renee A. Reijo Pera
CSH Protocols; 2008; doi:10.1101/pdb.prot5048
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Coimmunoprecipitation (co-IP) of Nuclear Proteins and Chromatin Immunoprecipitation (ChIP) from Arabidopsis
Berthe K. Fiil, Jin-Long Qiu, Klaus Petersen, Morten Petersen, and John Mundy
CSH Protocols; 2008; doi:10.1101/pdb.prot5049
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Information Panels

Antibody Staining of Drosophila Tissues--Helpful Hints
Ilaria Rebay and Richard G. Fehon
CSH Protocols; 2008; doi:10.1101/pdb.ip56
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Preparation of Baskets for Drosophila Egg Collections, Treatments, and Incubations
Antony W. Shermoen
CSH Protocols; 2008; doi:10.1101/pdb.ip57
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