Expression of Cloned Genes

Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 5: Expressing Proteins on NAPPA Slides
Andrew J. Link and Joshua LaBaer
CSH Protocols; 2008; doi:10.1101/pdb.prot5060 [Abstract] [Full text]

High-Throughput Cloning of Open Reading Frames (ORFs): Assembling Large Sets of Expression Constructs
Andrew J. Link and Joshua LaBaer
CSH Protocols; 2008; doi:10.1101/pdb.prot5050 [Abstract] [Full text]

The GAL4 System: A Versatile Toolkit for Gene Expression in Drosophila
Tony D. Southall, David A. Elliott, and Andrea H. Brand
CSH Protocols; 2008; doi:10.1101/pdb.top49 [Abstract] [Full text]

Generation of Driver and Reporter Constructs for the GAL4 Expression System in Drosophila
Tony D. Southall and Andrea H. Brand
CSH Protocols; 2008; doi:10.1101/pdb.prot5029 [Abstract] [Full text]

Culture of HEK293-EBNA1 Cells for Production of Recombinant Proteins
Roseanne Tom, Louis Bisson, and Yves Durocher
CSH Protocols; 2008; doi:10.1101/pdb.prot4976 [Abstract] [Full text]

Transfection of HEK293-EBNA1 Cells in Suspension with Linear PEI for Production of Recombinant Proteins
Roseanne Tom, Louis Bisson, and Yves Durocher
CSH Protocols; 2008; doi:10.1101/pdb.prot4977 [Abstract] [Full text]

Transfection of Adherent HEK293-EBNA1 Cells in a Six-Well Plate with Branched PEI for Production of Recombinant Proteins
Roseanne Tom, Louis Bisson, and Yves Durocher
CSH Protocols; 2008; doi:10.1101/pdb.prot4978 [Abstract] [Full text]

Transfection of HEK293-EBNA1 Cells in Suspension with 293fectin for Production of Recombinant Proteins
Roseanne Tom, Louis Bisson, and Yves Durocher
CSH Protocols; 2008; doi:10.1101/pdb.prot4979 [Abstract] [Full text]

Protein Nanospheres for Gene Delivery
Sushma Kommareddy and Mansoor M. Amiji
CSH Protocols; 2008; doi:10.1101/pdb.top30 [Abstract] [Full text]

Preparation and Loading of Gelatin Nanoparticles
Sushma Kommareddy and Mansoor M. Amiji
CSH Protocols; 2008; doi:10.1101/pdb.prot4885 [Abstract] [Full text]