Preparation of Macromolecules and Introduction into Cells

RNAi Experiments in Mouse Oocytes and Early Embryos
Petr Svoboda and Paula Stein
CSH Protocols; 2009; doi:10.1101/pdb.top56 [Abstract] [Full text]

Preparation of dsRNA for Microinjection Experiments in Mouse
Petr Svoboda
CSH Protocols; 2009; doi:10.1101/pdb.prot5131 [Abstract] [Full text]

Microinjection of dsRNA into Fully-Grown Mouse Oocytes
Paula Stein
CSH Protocols; 2009; doi:10.1101/pdb.prot5132 [Abstract] [Full text]

Microinjection of dsRNA into Mouse One-Cell Embryos
Paula Stein
CSH Protocols; 2009; doi:10.1101/pdb.prot5133 [Abstract] [Full text]

Cloning a Transgene for Transgenic RNAi in Mouse Oocytes
Petr Svoboda
CSH Protocols; 2009; doi:10.1101/pdb.prot5134 [Abstract] [Full text] [ Supplemental Material ]

Microinjection of Plasmids into Meiotically Incompetent Mouse Oocytes
Paula Stein
CSH Protocols; 2009; doi:10.1101/pdb.prot5135 [Abstract] [Full text]

Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version A
Gustavo Tiscornia, Oded Singer, and Inder M. Verma
CSH Protocols; 2008; doi:10.1101/pdb.prot5009 [Abstract] [Full text]

Design and Cloning of an shRNA into a Lentiviral Silencing Vector: Version B
Gustavo Tiscornia, Oded Singer, and Inder M. Verma
CSH Protocols; 2008; doi:10.1101/pdb.prot5010 [Abstract] [Full text]

Transduction of Cell Lines by Retroviral Vectors
Kenneth Cornetta, Karen E. Pollok, and A. Dusty Miller
CSH Protocols; 2008; doi:10.1101/pdb.prot4883 [Abstract] [Full text]

Transduction of Primary Hematopoietic Cells by Retroviral Vectors
Kenneth Cornetta, Karen E. Pollok, and A. Dusty Miller
CSH Protocols; 2008; doi:10.1101/pdb.prot4884 [Abstract] [Full text]