RNA Interference (RNAi)/siRNA

RNAi Experiments in Mouse Oocytes and Early Embryos
Petr Svoboda and Paula Stein
CSH Protocols; 2009; doi:10.1101/pdb.top56 [Abstract] [Full text]

Choosing the Sequence of dsRNA for RNAi in Mouse
Petr Svoboda
CSH Protocols; 2009; doi:10.1101/pdb.ip63 [Abstract] [Full text]

Cloning and Sequencing an Inverted Repeat
Petr Svoboda
CSH Protocols; 2009; doi:10.1101/pdb.ip64 [Abstract] [Full text]

Preparation of dsRNA for Microinjection Experiments in Mouse
Petr Svoboda
CSH Protocols; 2009; doi:10.1101/pdb.prot5131 [Abstract] [Full text]

Microinjection of dsRNA into Fully-Grown Mouse Oocytes
Paula Stein
CSH Protocols; 2009; doi:10.1101/pdb.prot5132 [Abstract] [Full text]

Microinjection of dsRNA into Mouse One-Cell Embryos
Paula Stein
CSH Protocols; 2009; doi:10.1101/pdb.prot5133 [Abstract] [Full text]

Cloning a Transgene for Transgenic RNAi in Mouse Oocytes
Petr Svoboda
CSH Protocols; 2009; doi:10.1101/pdb.prot5134 [Abstract] [Full text] [ Supplemental Material ]

Microinjection of Plasmids into Meiotically Incompetent Mouse Oocytes
Paula Stein
CSH Protocols; 2009; doi:10.1101/pdb.prot5135 [Abstract] [Full text]

Bone Marrow-Derived Macrophages (BMM): Isolation and Applications
Joachim Weischenfeldt and Bo Porse
CSH Protocols; 2008; doi:10.1101/pdb.prot5080 [Abstract] [Full text]

Gene Silencing by Injection and Electroporation of dsRNA in Avian Embryos
Olivier Mauti, Thomas Baeriswyl, and Esther T. Stoeckli
CSH Protocols; 2008; doi:10.1101/pdb.prot5094 [Abstract] [Full text]