Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4505

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Dissection of Spinal Cords for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos

Esther T. Stoeckli

This protocol was adapted from "RNAi in Avian Embryos," contributed by Esther T. Stoeckli, Chapter 14, in RNAi: A Guide to Gene Silencing (ed. Hannon). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.


INTRODUCTION

This protocol describes how to dissect spinal cords of dsRNA-treated avian embryos for analysis of commissural axon guidance. After fixation of the spinal cord with paraformaldehyde, commissural axons are stained with Fast-DiI.


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Related Protocol

Injection of dsRNA and Electroporation in Avian Embryos
Esther T. Stoeckli
Cold Spring Harb Protoc 2006: 4504. [Abstract] [Full Text]