Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4644

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Bradford Assay

Ed Harlow and David Lane

This protocol was adapted from "Protein Techniques," Appendix II, in Using Antibodies. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.


INTRODUCTION

This assay is used for protein quantitation. It is relatively accurate for most proteins, except for small basic polypeptides such as ribonuclease or lysozyme. It is also hampered by detergent concentrations over ~0.2%.


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