Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4614

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protocolProtocol

Freeze-Thaw Lysis for Extraction of Proteins from Mammalian Cells

William P. Tansey

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA

Corresponding author (tansey{at}cshl.edu)


INTRODUCTION

This protocol describes the extraction of proteins from mammalian cells grown in 6- or 10-cm tissue-culture dishes. It is useful for making very concentrated extracts for Western blotting, and is particularly helpful when low-abundance proteins such as Myc are being studied.


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