Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4652

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DNA Preparation and Needle Loading for Gene Delivery by Microinjection

David A. Dean

This protocol was adapted from "Gene Delivery by Direct Injection and Facilitation of Expression by Mechanical Stretch," Chapter 4, in Live Cell Imaging (eds. Goldman and Spector). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.


INTRODUCTION

For microinjection into cells, high-quality plasmid DNA is purified using standard procedures. The resulting preparation is then delivered into the microinjection needle by either a back-filling or a forward-filling approach.


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