Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4665

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Transformation of Agrobacterium Using Electroporation

Detlef Weigel and Jane Glazebrook

This protocol was adapted from "How to Transform Arabidopsis," Chapter 5, in Arabidopsis by Detlef Weigel and Jane Glazebrook. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2002.


INTRODUCTION

This protocol describes a method for transforming Agrobacterium with plasmid DNA using electroporation in a manner similar to that commonly used for Escherichia coli. Although the transformation efficiency for Agrobacterium is lower than that for E. coli, it is possible to obtain adequate numbers of Agrobacterium transformants with this technique.


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Y. Fang and D. L. Spector
Live Cell Imaging of Plants
Cold Spring Harb Protoc, February 1, 2010; 2010(2): pdb.top68 - pdb.top68.
[Abstract] [Full Text]