Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4641

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Pulse-Chase Assay for Measuring Protein Stability in Yeast

William P. Tansey

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA

Corresponding author (tansey{at}cshl.edu)


INTRODUCTION

This assay directly measures the stability of a specific protein in yeast. Following pulse-labeling of yeast cells, the target protein is recovered by denaturing immunoprecipitation.


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