Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4772

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protocolProtocol

Preparation of Zebrafish Embryos for Transmission Electron Microscopy

Sandra Rieger1 and Reinhard W. Köster

GSF-National Research Center for Environment and Health, Institute of Developmental Genetics, 85764 München-Neuherberg, Germany

1Corresponding author (sandra.rieger{at}gsf.de)


INTRODUCTION

This protocol describes a procedure for the fixation and embedding of zebrafish embryos at organogenesis stages (48-72 hours post-fertilization [hpf]) for transmission electron microscopy (TEM). The lengths of individual steps may be adjusted according to the developmental stage of the specimen.


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