Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4764

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schulz, C.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Schulz, C.
Related Collections
Right arrow Cell Biology, general
Right arrow Visualization
Right arrow Visualization, general
Right arrow Laboratory Organisms, general
Right arrow Drosophila
Right arrow Molecular Biology, general
Right arrow Analysis of Gene Expression
Right arrow Analysis of Gene Expression, general
Right arrow Probes, general
Right arrow Non-isotopically Labeled Probes
Right arrow RNA
Right arrow RNA, general
Right arrow mRNA
Right arrow Developmental Biology
Right arrow In Situ Hybridization
Right arrow Stem Cells
Right arrow Stem Cells, general
Right arrow Visualization of Gene Expression
Right arrow Polymerase Chain Reaction (PCR)
Right arrow Polymerase Chain Reaction (PCR), general
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
BSN globe

protocolProtocol

In Situ Hybridization to Drosophila Testes

Cordula Schulz

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA

Corresponding author (cschulz{at}cshl.edu)


INTRODUCTION

The tips of Drosophila testes harbor the germ line stem cells, their amplifying daughters, and the cells of the stem cell lineage environments. These cells, located within the apical 100 µm, are specifically hard to label, as the testes appear to be less penetrable at the tips. Here we report an in situ hybridization protocol that works reliably to detect RNA in the cells at the apical tips of Drosophila testes.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?