Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4828

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rothwell, W. F.
Right arrow Articles by Sullivan, W.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Rothwell, W. F.
Right arrow Articles by Sullivan, W.
Related Collections
Right arrow Genetics, general
Right arrow Imaging Development
Right arrow Laboratory Organisms, general
Right arrow Drosophila
Right arrow Immunohistochemistry
Right arrow Developmental Biology
Right arrow In Situ Hybridization
Right arrowRelated Protocols
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

protocolProtocol

Rehydration of Drosophila Embryos

Wendy F. Rothwell and William Sullivan

This protocol was adapted from "Fluorescent Analysis of Drosophila Embryos," Chapter 9, in Drosophila Protocols (eds. Sullivan et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2000.


INTRODUCTION

Fixed Drosophila embryos stored in methanol must be rehydrated before staining. Rehydration methods differ slightly for embryos fixed in methanol versus embryos fixed in formaldehyde; techniques for both are presented. An alternative, gentler rehydration method is also presented for studies where a new probe or antibody is being used.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Protocols

Drosophila Embryo Collection
Wendy F. Rothwell and William Sullivan
Cold Spring Harb Protoc 2007: 4825. [Abstract] [Full Text]

Drosophila Embryo Dechorionation
Wendy F. Rothwell and William Sullivan
Cold Spring Harb Protoc 2007: 4826. [Abstract] [Full Text]

Fixation of Drosophila Embryos
Wendy F. Rothwell and William Sullivan
Cold Spring Harb Protoc 2007: 4827. [Abstract] [Full Text]