Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4841

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protocolProtocol

Genotyping by Allele-Specific Amplification (KASPar)

Edwin Cuppen

Hubrecht Laboratorium, 3584 CT Utrecht, The Netherlands

Corresponding author (ecuppen{at}niob.knaw.nl)


INTRODUCTION

This protocol describes the use of allele-specific amplification followed by fluorescence detection (KASPar) for genotyping. The method is compatible with any real-time PCR setup and has the advantage that no specifically labeled oligonucleotides are needed.


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