Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4843

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Macdonald, S. J.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Macdonald, S. J.
Related Collections
Right arrow Bioinformatics/Genomics, general
Right arrow Genetics, general
Right arrow Genetic Variation
Right arrow Molecular Biology, general
Right arrow DNA Sequencing
Right arrow Genomic DNA
Right arrow Oligonucleotide Probes
Right arrow Genotypic Analysis
Right arrow Genome Analysis
Right arrow Microarrays
Right arrow Microarrays, general
Right arrow Genomic Analysis
Right arrow Generation of Probes for Microarrays
Right arrow Polymerase Chain Reaction (PCR)
Right arrow Polymerase Chain Reaction (PCR), general
Right arrow Amplification of DNA by PCR
Right arrow Detection of PCR Products
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
BSN globe

protocolProtocol

Genotyping by Oligonucleotide Ligation Assay (OLA)

Stuart J. Macdonald

Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas 66045, USA
Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045, USA

Corresponding author (sjmac{at}ku.edu)


INTRODUCTION

This protocol describes the oligonucleotide ligation assay (OLA), which uses a set of three oligonucleotides, in combination with a thermostable Taq DNA ligase enzyme, to discriminate single-nucleotide polymorphism (SNP) alleles. Sixteen-plex OLA genotyping reactions are carried out, and allele-specific OLA products are detected on membrane arrays using radiolabeled probes.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?