Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4850

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Chemical Two-Photon Uncaging

Diana L. Pettit and George J. Augustine

This protocol was adapted from "Practical Guide: Chemical Two-Photon Uncaging," Chapter 48, in Imaging in Neuroscience and Development (eds. Yuste and Konnerth). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.


INTRODUCTION

Chemical two-photon uncaging is useful for a wide range of applications, including both mapping of receptor location and localized photostimulation of neurons via activation of excitatory glutamate receptors. Experimental preparations could include brain slices, cultured neurons, and, among other possibilities, whole brains in vivo. This protocol documents the utility of chemical two-photon uncaging in examining glutamate receptors of pyramidal neurons in hippocampal slices.


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