Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4691

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Transgene Expression in Regenerated Roots

Jocelyn Malamy

This protocol was adapted from "How to Study Gene Function," Chapter 8, in Arabidopsis: A Laboratory Manual (eds. Weigel and Glazebrook). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2002.


INTRODUCTION

This procedure, which uses a root transformation protocol, provides a rapid method for assessing gene expression in Arabidopsis roots. It is useful for testing promoter:reporter gene constructs, for expressing genes, the overexpression of which is lethal in whole plants, and for transforming the roots of plants that are recalcitrant to conventional transformation techniques. The protocol has been used successfully with Ws, No-0, and RLD ecotypes.


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