Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4707

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protocolProtocol

Maintaining a Population of Drosophila

John Charles Sisson

This protocol was adapted from "Culturing Large Populations of Drosophila for Protein Biochemistry," Chapter 30, in Drosophila Protocols (eds. Sullivan et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2000.


INTRODUCTION

The Drosophila embryo is a superb source of proteins for studies aimed at characterizing a variety of cellular functions, including replication, transcription, translation, signal transduction, and the functions of the extracellular matrix and the cytoskeleton. The success of these studies requires the efficient production of gram quantities of embryos by large populations of adult flies. This protocol outlines a simple and efficient method for maintaining a population of Drosophila.


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