Cite as: Cold Spring Harb. Protoc.; 2007; doi:10.1101/pdb.prot4726

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Staining Frozen Mouse Embryo Sections for ß-Galactosidase (lacZ) Activity

Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer

This protocol was adapted from "Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems," Chapter 16, in Manipulating the Mouse Embryo, 3rd edition (eds. Nagy et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.


INTRODUCTION

Frozen mouse embryo sections are stained for ß-galactosidase (lacZ) activity using X-gal followed by counterstaining with Orange G.


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