Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot4699

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Simpson, R. J.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Simpson, R. J.
Related Collections
Right arrow Cell Biology, general
Right arrow Protein Identification and Analysis
Right arrow Molecular Biology, general
Right arrow Proteins and Proteomics, general
Right arrow Protein Classification and Structure Prediction
Right arrow Chromatography
Right arrow Chromatography, general
Right arrow Size-Exclusion Chromatography
Right arrowRelated Protocols
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
BSN globe

protocolProtocol

Estimation of Free Thiols and Disulfide Bonds Using Ellman’s Reagent

Richard J. Simpson

Adapted from Proteins and Proteomics by Richard J. Simpson. CSHL Press, Cold Spring Harbor, NY, USA, 2003.


INTRODUCTION

This protocol describes a method for quantitating the amount of free thiols and disulfide bond linkages in proteins. It uses the sulfhydryl reagent 5,5'-dithiobis (2-nitrobenzoic acid), or DTNB, which is also referred to as Ellman’s reagent.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Protocols

Desalting and Buffer Exchange of Proteins Using Size-Exclusion Chromatography
Helena Hedlund
CSH Protocols 2006: 4199. [Extract] [Full Text]

Reduction and S-Carboxymethylation of Proteins: Large-Scale Method
Richard J. Simpson
CSH Protocols 2007: 4569. [Abstract] [Full Text]

Quantifying Protein by Bicinchoninic Acid
Richard J. Simpson
CSH Protocols 2008: 4722. [Abstract] [Full Text]