Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot4804

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Subbing Slides

Carol Ann Paul, Barbara Beltz, and Joanne Berger-Sweeney

Adapted from Discovering Neurons: The Experimental Basis of Neuroscience (eds. Paul et al.). CSHL Press, Cold Spring Harbor, NY, USA, 1997.


INTRODUCTION

When mounting sections on a slide, it is essential that the slide be ultraclean. To ensure that the section sticks to the slide, slides can be covered with a thin layer of gelatin. The process of cleaning and gelatin-coating is called "subbing." Several boxes of slides should be subbed at a time and stored, covered, in the refrigerator until they are needed. Subbed slides can also be purchased commercially.


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C. A. Paul, B. Beltz, and J. Berger-Sweeney
The Nissl Stain: A Stain for Cell Bodies in Brain Sections
CSH Protocols, September 1, 2008; 2008(10): pdb.prot4805 - pdb.prot4805.
[Abstract] [Full Text]