Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot5000

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Streamlined Protocol for Antibody Staining of Large Numbers of Samples in Drosophila

Ilaria Rebay and Richard G. Fehon

Adapted from Drosophila Protocols (eds. Sullivan et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2000.


INTRODUCTION

The most common reason for generating antibodies against a particular protein in Drosophila is to examine its pattern of endogenous expression during development. Thus, once an antibody is shown to stain transfected cells, the next step is to try staining Drosophila tissues, usually embryos (described here), imaginal discs, or ovaries. This streamlined protocol can be used for processing a large number of samples, e.g., screening monoclonal antibodies.


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I. Rebay and R. G. Fehon
Antibody Staining of Drosophila Tissues--Helpful Hints
CSH Protocols, September 1, 2008; 2008(10): pdb.ip56 - pdb.ip56.
[Abstract] [Full Text]