Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot5044

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Culturing Human Embryonic Stem Cells in Feeder-Free Conditions

Sohyun L. McElroy1 and Renee A. Reijo Pera

Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University, Palo Alto, CA 94304-5542, USA

1Corresponding author (sohyun{at}stanford.edu)


INTRODUCTION

Human embryonic stem cells (hESCs) have the potential to differentiate into all three germ layers and proliferate in long-term culture in vitro. hESCs can provide a cell source for the testing of novel therapies, drug screening, and functional genomics applications. Undifferentiated hESCs can be maintained and proliferated on mouse embryonic fibroblasts (MEFs) or human feeder cells. In this protocol, we describe the culture of hESCs in feeder-free conditions on Matrigel with MEF-conditioned medium. This protocol can be used for applications such as genetic modification of hESCs without feeder cell contamination.


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