Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot5054

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Gene Knockdown in Planarians Using RNA Interference

Néstor J. Oviedo, Cindy L. Nicolas, Dany S. Adams, and Michael Levin1

Center for Regenerative and Developmental Biology, Forsyth Institute and Developmental Biology Department, Harvard School of Dental Medicine, Boston, MA 02115, USA

1Corresponding author (mlevin{at}forsyth.org)


INTRODUCTION

This protocol describes how to produce gene knockdown in planarians using RNA interference (RNAi). It is a standard technique to evaluate gene function during regeneration and tissue maintenance in planarians. The procedure involves microinjecting double-stranded RNA (dsRNA) synthesized in vitro. Depending on the gene target, this technique can produce robust phenotypes that can be further evaluated by diverse macroscopic or microscopic procedures.


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This article has been cited by other articles:


Home page
 CSH ProtocolsHome page
N. J. Oviedo, C. L. Nicolas, D. S. Adams, and M. Levin
Planarians: A Versatile and Powerful Model System for Molecular Studies of Regeneration, Adult Stem Cell Regulation, Aging, and Behavior
CSH Protocols, October 1, 2008; 2008(11): pdb.emo101 - pdb.emo101.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
N. J. Oviedo, C. L. Nicolas, D. S. Adams, and M. Levin
Establishing and Maintaining a Colony of Planarians
CSH Protocols, October 1, 2008; 2008(11): pdb.prot5053 - pdb.prot5053.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
N. J. Oviedo, C. L. Nicolas, D. S. Adams, and M. Levin
Live Imaging of Planarian Membrane Potential Using DiBAC4(3)
CSH Protocols, October 1, 2008; 2008(11): pdb.prot5055 - pdb.prot5055.
[Abstract] [Full Text]