Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot5055

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Live Imaging of Planarian Membrane Potential Using DiBAC4(3)

Néstor J. Oviedo, Cindy L. Nicolas, Dany S. Adams, and Michael Levin1

Center for Regenerative and Developmental Biology, Forsyth Institute and Developmental Biology Department, Harvard School of Dental Medicine, Boston, MA 02115, USA

1Corresponding author (mlevin{at}forsyth.org)


INTRODUCTION

This protocol describes how to use the anionic membrane voltage-reporting dye DiBAC4(3) to generate images of cell membrane potential in live planarians. These images qualitatively reveal variations in time-averaged membrane potential across different regions of the organism. Changes in these images due to experimental treatments reveal how the particular treatment affects this physiological parameter. This method is a great improvement over standard electrophysiological techniques, which cannot be used to gain an understanding of the electrical properties of an entire worm or a regenerating fragment, due to small cell size and large cell number. When the proper controls are performed, this technique is a very powerful and simple way to gather physiologic data.


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This article has been cited by other articles:


Home page
 CSH ProtocolsHome page
N. J. Oviedo, C. L. Nicolas, D. S. Adams, and M. Levin
Planarians: A Versatile and Powerful Model System for Molecular Studies of Regeneration, Adult Stem Cell Regulation, Aging, and Behavior
CSH Protocols, October 1, 2008; 2008(11): pdb.emo101 - pdb.emo101.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
N. J. Oviedo, C. L. Nicolas, D. S. Adams, and M. Levin
Establishing and Maintaining a Colony of Planarians
CSH Protocols, October 1, 2008; 2008(11): pdb.prot5053 - pdb.prot5053.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
N. J. Oviedo, C. L. Nicolas, D. S. Adams, and M. Levin
Gene Knockdown in Planarians Using RNA Interference
CSH Protocols, October 1, 2008; 2008(11): pdb.prot5054 - pdb.prot5054.
[Abstract] [Full Text]