Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot5063

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Isolation of Pristionchus Nematodes from Beetles

Robbie Rae, Benjamin Schlager, and Ralf J. Sommer1

Max-Planck Institute for Developmental Biology, Department of Evolutionary Biology, D-72076 Tübingen, Germany

1Corresponding author (ralf.sommer{at}tuebingen.mpg.de)


INTRODUCTION

In this procedure, nematodes disembark from a beetle carcass and feed on Escherichia coli OP50. The nematodes are then monitored for a few days and identified using simple morphological characteristics. This method is rapid, easy, and biased for Pristionchus species.


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This article has been cited by other articles:


Home page
 CSH ProtocolsHome page
R. Rae, B. Schlager, and R. J. Sommer
Pristionchus pacificus: A Genetic Model System for the Study of Evolutionary Developmental Biology and the Evolution of Complex Life-History Traits
CSH Protocols, October 1, 2008; 2008(11): pdb.emo102 - pdb.emo102.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
R. Rae, B. Schlager, and R. J. Sommer
Assessment of the Olfactory Response to Chemicals or Bacteria in Pristionchus Nematodes
CSH Protocols, October 1, 2008; 2008(11): pdb.prot5064 - pdb.prot5064.
[Abstract] [Full Text]