Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot5079

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Sex Chromatin Staining in Amnion Cells

Blanche Capel1 and Jordan Batchvarov

Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA

1 Corresponding author (b.capel{at}cellbio.duke.edu)


INTRODUCTION

The procedure described here provides a quick and reliable method for determining the sex of mouse embryos that are <12.5 days post-coitum (dpc). Cells from amniotic membranes are stained with toluidine blue. The presence of a heavily stained condensed chromatin body (i.e., a Barr body) indicates the XX samples. With experience, we find a >95% concordance with genotyping data based on PCR for Y-chromosome sequences in extracted tail DNA. This protocol has the advantage of speed and efficiency: When assembling cultures with live tissue, samples can be sexed in 30 min.


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B. Capel and J. Batchvarov
Preparing Recombinant Gonad Organ Cultures
CSH Protocols, November 1, 2008; 2008(12): pdb.prot5078 - pdb.prot5078.
[Abstract] [Full Text]