Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot5092

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In Vitro Fertilization of Astyanax mexicanus

Richard Borowsky

Department of Biology, New York University, NY 10003, USA

Corresponding author (rborowsky{at}nyu.edu)


INTRODUCTION

This protocol describes an alternative method for breeding Astyanax mexicanus, using in vitro fertilization. Sperm collected from the male and eggs collected from the female are placed in a Petri dish, and sperm are activated by the addition of fresh system water. The eggs are observed under low magnification for signs of fertilization, usually marked by the onset of cleavage.


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Related Article

Astyanax mexicanus, the Blind Mexican Cave Fish: A Model for Studies in Development and Morphology
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This article has been cited by other articles:


Home page
 CSH ProtocolsHome page
R. Borowsky
Astyanax mexicanus, the Blind Mexican Cave Fish: A Model for Studies in Development and Morphology
CSH Protocols, November 1, 2008; 2008(12): pdb.emo107 - pdb.emo107.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
R. Borowsky
Breeding Astyanax mexicanus through Natural Spawning
CSH Protocols, November 1, 2008; 2008(12): pdb.prot5091 - pdb.prot5091.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
R. Borowsky
Handling Astyanax mexicanus Eggs and Fry
CSH Protocols, November 1, 2008; 2008(12): pdb.prot5093 - pdb.prot5093.
[Abstract] [Full Text]