Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot5096

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protocolProtocol

Whole-Mount In Situ Hybridization in Amphimedon

Claire Larroux1, Bryony Fahey1, Maja Adamska1,2, Gemma S. Richards1, Marie Gauthier1, Kathryn Green1, Erica Lovas1, and Bernard M. Degnan1,3

1 School of Integrative Biology, University of Queensland, Brisbane QLD 4072, Australia
2 Sars International Centre for Marine Molecular Biology, N-5008 Bergen, Norway

3Corresponding author (b.degnan{at}uq.edu.au)


INTRODUCTION

Developmental gene expression is analyzed predominantly via whole-mount in situ hybridization using digoxigenin-labeled RNA probes. This protocol describes how to perform this procedure in Amphimedon queenslandica, including fixation, hybridization, and sectioning of embryonic, larval, and post-larval juvenile stages.


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This article has been cited by other articles:


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 Cold Spring Harb ProtocHome page
B. M. Degnan, M. Adamska, A. Craigie, S. M. Degnan, B. Fahey, M. Gauthier, J. N.A. Hooper, C. Larroux, S. P. Leys, E. Lovas, et al.
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 Cold Spring Harb ProtocHome page
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