Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot5109

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Using the Nucleic Acid Programmable Protein Array (NAPPA) for Identifying Protein-Protein Interactions. Protocol 2: Detection of Query Proteins on NAPPA Slides

Andrew J. Link1 and Joshua LaBaer2,3

1 Vanderbilt University School of Medicine, Nashville, TN 37232-8575, USA
2 Harvard University School of Medicine, Harvard Institute of Proteomics, Cambridge, MA 02141-2023, USA

3Corresponding author (joshua_labaer{at}hms.harvard.edu)


INTRODUCTION

The Nucleic Acid Programmable Protein Array (NAPPA) approach for producing protein microarrays uses cell-free extracts to transcribe and translate cDNAs encoding target proteins directly onto glass slides. Identification of protein interactions can be accomplished either by probing an expressed NAPPA slide with a purified protein of interest (the query protein) or by coexpressing the query protein on the NAPPA slide at the same time that the target proteins are expressed. This protocol describes detection of query protein following coexpression of the query protein on the NAPPA array.


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Related Article

Using the Nucleic Acid Programmable Protein Array (NAPPA) for Identifying Protein-Protein Interactions: General Guidelines
Andrew J. Link and Joshua LaBaer
Cold Spring Harb Protoc 2008: 62. [Abstract] [Full Text]

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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
A. J. Link and J. LaBaer
Using the Nucleic Acid Programmable Protein Array (NAPPA) for Identifying Protein-Protein Interactions: General Guidelines
Cold Spring Harb Protoc, December 1, 2008; 2008(13): pdb.ip62 - pdb.ip62.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
A. J. Link and J. LaBaer
Using the Nucleic Acid Programmable Protein Array (NAPPA) for Identifying Protein-Protein Interactions. Protocol 1: Coexpression of Query Protein on NAPPA Slides
Cold Spring Harb Protoc, December 1, 2008; 2008(13): pdb.prot5108 - pdb.prot5108.
[Abstract] [Full Text]